Mutations found in the pncA gene of Mycobacterium tuberculosis in clinical pyrazinamide-resistant isolates from a local region of China

The Journal of International Medical Research
H ZhangXian-En Zhang

Abstract

This study was designed to investigate the presence of mutations in the pncA gene, minimum inhibitory concentrations and pyrazinamidase activity of pyrazinamide-resistant Mycobacterium tuberculosis. In total, 47 M. tuberculosis clinical isolates from a local region of China were assayed. Pyrazinamidase activity was measured by pyrazinamide deamination to pyrazinoic acid and ammonia, and a 721 bp region, including the entire pncA open-reading frame, 104 bp of the upstream sequence and 59 bp of the downstream sequence, was determined by DNA sequencing of purified polymerase chain reaction products. Of the 47 isolates resistant to pyrazinamide, 44 lost pyrazinamidase activity and had pncA mutations that occurred mainly near pyrazinamidase's active or metal ion binding sites; nine of them have not been reported previously. Three pyrazinamide-resistant isolates carried the wild-type pncA sequence and retained pyrazinamidase activity. These results show the molecular mechanism of pyrazinamide resistance in China and may also contribute towards the prevention of tuberculosis in China.

References

May 1, 1992·The American Review of Respiratory Disease·L Heifets, P Lindholm-Levy
Mar 1, 1997·Antimicrobial Agents and Chemotherapy·A ScorpioY Zhang
Mar 1, 1997·Antimicrobial Agents and Chemotherapy·S SreevatsanJ M Musser
Jul 2, 1999·Antimicrobial Agents and Chemotherapy·H J MarttilaM K Viljanen
Aug 22, 2000·Antimicrobial Agents and Chemotherapy·G P MorlockR C Cooksey
Feb 7, 2001·Journal of Clinical Microbiology·M M HannanJ T Crawford
Jun 29, 2001·BMC Infectious Diseases·S K ParkS H Jeong
Oct 20, 2001·Journal of Korean Medical Science·K W LeeK S Jung
Feb 5, 2002·Journal of Clinical Microbiology·Yasuhiko SuzukiHajime Oda
Oct 25, 2003·Antimicrobial Agents and Chemotherapy·Tsi-Shu HuangYung-Ching Liu
Feb 20, 2004·Diagnostic Microbiology and Infectious Disease·Chika MiyagiTetsuya Takashima
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·Isabel PortugalLaura Brum
Dec 24, 2004·Antimicrobial Agents and Chemotherapy·Vívian de F Sumnienski RodriguesArnaldo Zaha
Jan 19, 2008·The FEBS Journal·Hua ZhangXian-En Zhang

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Citations

Oct 1, 2010·Biochemistry·Derrick R SeinerJohn S Blanchard
Jan 17, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Betzaida Cuevas-CórdobaRoberto Zenteno-Cuevas
Jul 29, 2015·PloS One·Michael G WhitfieldAnnelies van Rie
Nov 2, 2015·Advances in Medical Sciences·Moses NjireTianyu Zhang
Jul 28, 2017·The American Journal of Tropical Medicine and Hygiene·Sushim Kumar GuptaJean-Marc Rolain
Jun 17, 2015·Antimicrobial Agents and Chemotherapy·Sarah M Ramirez-Busby, Faramarz Valafar
Sep 18, 2015·Journal of Clinical Microbiology·Nontuthuko E ManingiP Bernard Fourie
Jul 20, 2011·Antimicrobial Agents and Chemotherapy·Kwok Chiu ChangYing Zhang
Apr 24, 2020·Biotechnology Letters·Chuang YanGuimin Zhang
Nov 4, 2021·Biotechnology and Applied Biochemistry·Nasir MahmoodSarah Bushra Nasir

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