Nov 6, 2018

Mutations in bacterial genes induce unanticipated changes in the relationship between bacterial pathogens in experimental otitis media

BioRxiv : the Preprint Server for Biology
Vinal LakhaniJayajit Das

Abstract

Otitis media (OM) is a common polymicrobial infection of the middle ear in children under the age of fifteen years. A widely used experimental strategy to analyze roles of specific phenotypes of bacterial pathogens of OM is to study changes in co-infection kinetics of bacterial populations in animal models when a wild type bacterial strain is replaced by a specific isogenic mutant strain in the co-inoculating mixtures. Since relationships between the OM bacterial pathogens within the host are regulated by many interlinked processes, connecting the changes in the co-infection kinetics to a bacterial phenotype can be challenging. We investigated middle ear co-infections in adult chinchillas (Chinchilla lanigera) by two major OM pathogens: nontypeable Haemophilus influenzae (NTHi) and Moraxella catarrhalis (Mcat), as well as isogenic mutant strains in each bacterial species. We analyzed the infection kinetic data using Lotka-Volterra population dynamics, Maximum Entropy inference, and Akaike Information Criteria (AIC) based model selection. We found that changes in relationships between the bacterial pathogens that were not anticipated in the design of the co-infection experiments involving mutant strains are common and were stron...Continue Reading

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Chinchilla lanigera
Disorder of Middle Ear
Malignant Neoplasm of Middle Ear
Entire Middle Ear
Size
Haemophilus influenzae
Coinfection
Right Middle Ear Structure
Osteomyelitis
MCAT gene

About this Paper

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.