Mutations in cell division cycle genes CDC36 and CDC39 activate the Saccharomyces cerevisiae mating pheromone response pathway.

Molecular and Cellular Biology
M de Barros LopesS I Reed

Abstract

Conditional mutations in the genes CDC36 and CDC39 cause arrest in the G1 phase of the Saccharomyces cerevisiae cell cycle at the restrictive temperature. We present evidence that this arrest is a consequence of a mutational activation of the mating pheromone response. cdc36 and cdc39 mutants expressed pheromone-inducible genes in the absence of pheromone and conjugated in the absence of a mating pheromone receptor. On the other hand, cells lacking the G beta subunit or overproducing the G alpha subunit of the transducing G protein that couples the receptor to the pheromone response pathway prevented constitutive activation of the pathway in cdc36 and cdc39 mutants. These epistasis relationships imply that the CDC36 and CDC39 gene products act at the level of the transducing G protein. The CDC36 and CDC39 gene products have a role in cellular processes other than the mating pheromone response. A mating-type heterozygous diploid cell, homozygous for either the cdc36 or cdc39 mutation, does not exhibit the G1 arrest phenotype but arrests asynchronously with respect to the cell cycle. A similar asynchronous arrest was observed in cdc36 and cdc39 cells where the pheromone response pathway had been inactivated by mutations in the tr...Continue Reading

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Citations

Mar 16, 2012·Critical Reviews in Biochemistry and Molecular Biology·Jason E Miller, Joseph C Reese
Oct 1, 1991·Current Opinion in Genetics & Development·S I Reed
Oct 25, 1995·Molecular & General Genetics : MGG·Y Tange, O Niwa
Aug 1, 1992·Antonie van Leeuwenhoek·J B Konopka, S Fields
Jul 5, 2003·The Journal of Biological Chemistry·Pamela K HansonJ Wylie Nichols
Jul 3, 2007·PLoS Pathogens·Boris StriepenGiel G van Dooren
Feb 15, 1994·FEMS Microbiology Letters·D R EvansA E Wheals
Apr 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Guokun WangDina Petranovic
Nov 4, 2020·Cells·Nafiseh Chalabi Hagkarim, Roger J Grand
Feb 16, 1991·Biochimica Et Biophysica Acta·J W Dolan, S Fields

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