Mutations in central carbon metabolism genes suppress defects in nucleoid position and cell division of replication mutants in Escherichia coli

Gene
Monika Maciąg-DorszyńskaAgnieszka Szalewska-Pałasz

Abstract

A genetic link of the carbon metabolism and DNA replication was recently reported for the representative of Gram-negative bacteria, Escherichia coli. Our studies showed that the viability of thermosensitive replication mutants at high temperature can be improved or fully recovered by deleting certain genes of central carbon metabolism (CCM). In order to improve our understanding of this phenomenon, in this study we analyzed the length and nucleoid distribution of suppressed thermosensitive replication mutants. The dysfunctions in the replication machinery generally lead to formation of elongated cells (termed filaments) that originate from an inhibition of cell division dependent on replication-stress, and to abnormal distribution and compaction of nucleoids. The results reported here provide evidence that deletion of the pta and ackA CCM genes significantly reduces observed cell length in the replication mutants dnaA46, dnaB8, dnaE486, dnaG(ts) and dnaN159. A weaker effect was shown in the tktB dnaE486 double mutant. The CCM enzyme dysfunction restored also the nucleoid shape and position in double mutants. The specificity of these effects was confirmed by overexpression of fully functional genes coding for relevant CCM enzyme...Continue Reading

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Citations

Oct 24, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Eric H HillDavid G Whitten
Jan 1, 2012·Life·Vic Norris, Patrick Amar
Sep 27, 2018·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Hamid NouriLaurent Janniere
Sep 30, 2020·FEMS Microbiology Reviews·Alix MeunierManuel Campos
Jun 3, 2021·International Journal of Molecular Sciences·Lidia BossAgnieszka Szalewska-Pałasz

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