PMID: 11930117Apr 4, 2002Paper

Mutations in exon 3 of the beta-catenin gene are rare in melanoma cell lines

Melanoma Research
P M Pollock, N K Hayward

Abstract

Mutations in exon 3 of the CTNNB1 gene encoding beta-catenin have been reported in colorectal cancer cell lines and tumours. Although one study reported mutations or deletions affecting beta-catenin in 20% of melanoma cell lines, subsequent reports detected a much lower frequency of aberrations in uncultured melanomas. To determine whether this difference in mutation frequency reflected an in vitro culturing artefact, exon 3 of CTNNB1 was screened in a panel of 62 melanoma cell lines. In addition, reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect intragenic deletions affecting exon 3. One out of 62 (1.6%) cell lines was found to carry a mutation, indicating that aberration of the Wnt-1/wingless pathway through activation of beta-catenin is a rare event, even in melanoma cell lines.

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Citations

Jun 30, 2005·Cancer Metastasis Reviews·Ashani T Weeraratna
Jun 5, 2003·Biochimica Et Biophysica Acta·Rachel H GilesHans Clevers
Jan 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andy J ChienRandall T Moon
Oct 18, 2005·Biochimica Et Biophysica Acta·Joydeb Kumar KunduYoung-Joon Surh
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Sep 15, 2004·Cancer Letters·Monica RodolfoViviana Vallacchi
Nov 29, 2007·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Christina Dahl, Per Guldberg
Apr 4, 2008·Genes, Chromosomes & Cancer·Daniele CastigliaStefania D'Atri
Nov 10, 2007·Melanoma Research·Wieke ZuidervaartNicholas K Hayward
Sep 23, 2009·The Journal of Pathology·Silke Kuphal, Anja Bosserhoff
Mar 20, 2010·Genetika·V A TronovL B Gorbacheva
Apr 23, 2003·Journal of Cancer Research and Clinical Oncology·B Lustig, J Behrens
Nov 1, 2017·Virchows Archiv : an International Journal of Pathology·B T TeunissenW A M Blokx
Jul 15, 2020·International Journal of Molecular Sciences·Anna Gajos-Michniewicz, Malgorzata Czyz
Sep 13, 2008·Cell Stem Cell·Richard Mark White, Leonard I Zon

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