Mutations in gyrB play an important role in ciprofloxacin-resistant Pseudomonas aeruginosa

Infection and Drug Resistance
Xinyuan FengJinju Duan

Abstract

To investigate the main molecular resistance mechanisms to fluoroquinolones (FQs) in Pseudomonas aeruginosa and also to investigate the effect of time and concentration on mutations in resistance genes. The clinical isolates of P. aeruginosa which are sensitive to ciprofloxacin (CIP) or levofloxacin (LEV) were collected. The isolates were incubated with different concentrations of CIP or LEV for 5 days and the minimal inhibitory concentrations (MICs) of CIP, LEV and ofloxacin (OFX) were measured. The MIC of FQs to P. aeruginosa was measured by the agar dilution method. FQ resistance determining regions of gyrA, gyrB, parC and parE were amplified by PCR, and mutations in four genes were explored using sequence analysis with the Snapgene software. The relative expression levels of two efflux pumps genes (mexA and mexE) were measured by quantitative reverse transcription PCR. A total of eleven isolates were collected from the Second Hospital of Shanxi Medical University. Amino acid alterations in gyrA and gyrB were mainly detected in resistant mutants, and the percentage of strains with amino acid alterations in gyrB was significantly higher than that in gyrA (P<0.001). MICs of strains with mutations both in gyrA and gyrB were not...Continue Reading

Citations

Aug 9, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Bruno C BoettgerAntonio C C Pignatari
Feb 3, 2021·Critical Reviews in Microbiology·Susana Patrícia LopesMaria Olívia Pereira
Feb 18, 2021·Antimicrobial Agents and Chemotherapy·Dmitrii I ShiriaevOlga A Dontsova
Jul 13, 2021·Frontiers in Cellular and Infection Microbiology·Morgan A AlfordRobert E W Hancock

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Datasets Mentioned

BETA
879230
879741
879897

Methods Mentioned

BETA
PCR
reverse transcription PCR

Software Mentioned

Snapgene

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