PMID: 9192628Jun 24, 1997Paper

Mutations in HIV reverse transcriptase which alter RNase H activity and decrease strand transfer efficiency are suppressed by HIV nucleocapsid protein

Proceedings of the National Academy of Sciences of the United States of America
C E CameronS J Benkovic

Abstract

Structural studies of authentic HIV reverse transcriptase (RT) suggest a role for the p51 carboxyl terminus in forming an active RNase H conformation [Rodgers, D. W., Gamblin, S. J., Harris, B. A., Ray, S., Culp, J. S., Hellmig, B., Woolf, D. J., Debouck, C. & Harrison, S. C. (1995) Proc. Natl. Acad. Sci. USA 92, 1222-1226]. We have purified mutant RT heterodimers containing deletion of 5, 9, or 13 amino acids from the p51 carboxyl terminus. These "selectively deleted" heterodimers have been analyzed for changes in RNA-dependent DNA polymerase activity, RNase H activity, and the ability to catalyze DNA strand transfer. As deletions extended into the p51 subunit, a decrease in the stability of the RT-DNA complex was apparent. The largest effect was observed for p66/p51Delta13 RT, which showed a 3-fold decrease relative to wild-type RT. RNase H activity was measured by digestion of the RNA in a 5' 32P-labeled RNA/DNA hybrid. Deletion of 5 or 9 amino acids from p51 had little effect on synthesis-dependent and synthesis-independent RNase H activities. In contrast, deletion of 13 amino acids from p51 increased the length of the hydrolysis products of both RNase H activities by 8-10 bp, thus changing the spatial relationship between ...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·V GopalakrishnanS J Benkovic
Dec 8, 1995·Journal of Molecular Biology·J L DarlixB P Roques
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·D W RodgersS C Harrison
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W MetzgerH Heumann

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Citations

Mar 30, 1999·Biochimica Et Biophysica Acta·B I KlasensB Berkhout
Jan 15, 2013·Nature Structural & Molecular Biology·Mikalai LapkouskiWei Yang
Aug 25, 2000·AIDS Research and Human Retroviruses·H De RocquignyB P Roques
Nov 25, 1998·Nucleic Acids Research·R E JeeningaB Berkhout
Jul 20, 2011·Antimicrobial Agents and Chemotherapy·Richard V FariasOscar Leon
Feb 28, 2002·Journal of Virology·M Henrietta Nymark-McMahonSuzanne B Sandmeyer
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Adewunmi Onafuwa-Nuga, Alice Telesnitsky
Oct 6, 1998·Annual Review of Biochemistry·A D Frankel, J A Young
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·M Negroni, H Buc
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S DruillennecM C Fournié-Zaluski
Oct 29, 2011·Cellular and Molecular Life Sciences : CMLS·Bin XueVladimir N Uversky
Oct 12, 2005·Journal of Molecular Biology·Mark Nils HansonRobert A Bambara
Nov 9, 2001·Annual Review of Genetics·M Negroni, H Buc
Jul 1, 2016·Microbiology and Molecular Biology Reviews : MMBR·Guangdi Li, Erik De Clercq
Jul 25, 2003·The Journal of Biological Chemistry·Yan ChenRobert A Bambara
Jan 20, 2005·The Journal of Biological Chemistry·Yan ChenRobert A Bambara
Mar 6, 2007·The Journal of Biological Chemistry·Vandana PurohitRobert A Bambara
Apr 19, 2013·The Journal of Biological Chemistry·Suhman ChungStuart F J Le Grice
Nov 19, 2013·Pharmaceutical Patent Analyst·Mitesh PatelAshim K Mitra
Apr 10, 1999·The Journal of Biological Chemistry·S DruillennecB P Roques
May 8, 2002·The Journal of Biological Chemistry·Daniela LenerStuart F J Le Grice

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