PMID: 9188554Jul 1, 1997Paper

Mutations in reovirus outer-capsid protein sigma3 selected during persistent infections of L cells confer resistance to protease inhibitor E64

Journal of Virology
G S Baer, T S Dermody

Abstract

Mutations selected in reoviruses isolated from persistently infected cultures (PI viruses) affect viral entry into cells. Unlike wild-type (wt) viruses, PI viruses can grow in the presence of ammonium chloride, a weak base that blocks acid-dependent proteolysis of viral outer-capsid proteins in cellular endosomes during viral entry. In this study, we show that E64, an inhibitor of cysteine proteases such as those present in the endocytic compartment, blocks growth of wt reovirus by inhibiting viral disassembly. To determine whether PI viruses can grow in the presence of an inhibitor of endocytic proteases, we compared yields of wt and PI viruses in cells treated with E64. Prototype PI viruses L/C, PI 2A1, and PI 3-1 produced substantially greater yields than wt viruses type 1 Lang (T1L) and type 3 Dearing (T3D) in E64-treated cells. To identify viral genes that segregate with growth of PI viruses in the presence of E64, we tested reassortant viruses isolated from independent crosses of T1L and each of the prototype PI viruses for growth in cells treated with E64. Growth of reassortant viruses in the presence of E64 segregated exclusively with the S4 gene, which encodes viral outer-capsid protein sigma3. These results suggest th...Continue Reading

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Citations

Jan 19, 2002·Journal of Virology·Jodi L Connolly, Terence S Dermody
Jan 25, 2008·Journal of Virology·Melissa S MaginnisTerence S Dermody
Jul 31, 2009·Journal of Virology·Elizabeth M JohnsonTerence S Dermody
Oct 15, 2010·Journal of Virology·Payel Sarkar, Pranav Danthi
Nov 11, 2011·Journal of Virology·Rachel M NygaardLeslie A Schiff
May 2, 2020·The Journal of Cell Biology·Isabel Fernández de CastroCristina Risco
Jul 5, 2002·Journal of Virology·Joseph W GoldenLeslie A Schiff
Oct 31, 2003·The Journal of Biological Chemistry·Daniel H EbertTerence S Dermody
Feb 17, 2007·The Journal of Biological Chemistry·Pierre SchellingThilo Stehle
Sep 27, 2013·Journal of Virology·Andrea J PruijssersTerence S Dermody
Jun 26, 2015·Journal of Virology·Bernardo A MainouTerence S Dermody
Nov 23, 2016·The Journal of Biological Chemistry·Anthony J Snyder, Pranav Danthi
Jul 25, 2020·The Journal of General Virology·Hayato HarimaHirofumi Sawa
Feb 28, 2006·Journal of Virology·Melissa S MaginnisTerence S Dermody
Jun 20, 2001·Journal of Virology·M Mochow-Grundy, T S Dermody
Oct 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Melina A AgostoMax L Nibert
Jan 19, 2012·The Journal of Biological Chemistry·Joshua D DoyleTerence S Dermody
Dec 25, 2015·Journal of Virology·Anthony J Snyder, Pranav Danthi
Oct 3, 2019·Nature Communications·Melanie KoehlerDavid Alsteens
Feb 29, 2020·PLoS Pathogens·Pavithra AravamudhanTerence S Dermody
May 3, 2002·The Journal of Biological Chemistry·Daniel H EbertTerence S Dermody
Dec 13, 2003·The Journal of Biological Chemistry·Joseph W GoldenLeslie A Schiff
Jan 21, 2011·Journal of Virology·Bernardo A Mainou, Terence S Dermody
Oct 26, 2007·Journal of Virology·Pranav DanthiTerence S Dermody
Jun 21, 2011·Annual Review of Microbiology·Takamasa InoueBilly Tsai

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