Aug 5, 1989

Mutations in ribosomal proteins S4 and S12 influence the higher order structure of 16 S ribosomal RNA

Journal of Molecular Biology
P N Allen, H F Noller

Abstract

We have studied the effects of protein mutations on the higher order structure of 16 S rRNA in Escherichia coli ribosomes, using a set of structure-sensitive chemical probes. Ten mutant strains were studied, which contained alterations in ribosomal proteins S4 and S12, including double mutants containing both altered S4 and S12. Two ribosomal ambiguity (ram) S4 mutant strains, four streptomycin resistant (SmR) S12 mutant strains, one streptomycin pseudodependent (SmP) S12 mutant strain, one streptomycin dependent (SmD) S12 mutant strain and two streptomycin independent (Sm1) double mutants (containing both-SmD and ram mutations) were probed and compared to an isogenic wild-type strain. In ribosomes from strains containing S4 ram mutations, nucleotides A8 and A26 become more reactive to dimethyl sulfate (DMS) at their N-1 positions. In ribosomes from strains bearing the SmD allele, A908, A909, A1413 and G1487 are significantly less reactive to chemical probes. These same effects are observed when the S4 and S12 mutations are present simultaneously in the double mutants. An interesting correlation is found between the reactivity of A908 and the miscoding potential of SmR, SmD, SmP and wild-type ribosomes; the reactivity of A908 i...Continue Reading

  • References18
  • Citations62

References

Mentioned in this Paper

Bacterial Proteins
Alkalescens-Dispar Group
Ribosomal Proteins
MT-TA gene
Triplet Codon-amino Acid Adaptor Activity
Ribosomal protein S4
Higher Order Chromatin Structure
Transfer RNA
SNRPN
Ribosomal RNA

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