PMID: 9557647Apr 29, 1998Paper

Mutations in rotavirus nonstructural glycoprotein NSP4 are associated with altered virus virulence

Journal of Virology
M ZhangM K Estes

Abstract

Rotaviruses are major pathogens causing life-threatening dehydrating gastroenteritis in children and animals. One of the nonstructural proteins, NSP4 (encoded by gene 10), is a transmembrane, endoplasmic reticulum-specific glycoprotein. Recently, our laboratory has shown that NSP4 causes diarrhea in 6- to 10-day-old mice by functioning as an enterotoxin. To confirm the role of NSP4 in rotavirus pathogenesis, we sequenced gene 10 from two pairs of virulent and attenuated porcine rotaviruses, the OSU and Gottfried strains. Comparisons of the NSP4 sequences from these two pairs of rotaviruses suggested that structural changes between amino acids (aa) 131 and 140 are important in pathogenesis. We next expressed the cloned gene 10 from the OSU virulent (OSU-v) and OSU attenuated (OSU-a) viruses by using the baculovirus expression system and compared the biological activities of the purified proteins. NSP4 from OSU-v virus increased intracellular calcium levels over 10-fold in intestinal cells when added exogenously and 6-fold in insect cells when expressed endogenously, whereas NSP4 from OSU-a virus had little effect. NSP4 from OSU-v caused diarrhea in 13 of 23 neonatal mice, while NSP4 from OSU-a caused disease in only 4 of 25 mice...Continue Reading

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Citations

Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·K KunzelmannD I Cook
Sep 12, 2000·Current Gastroenterology Reports·R W Goodgame
Oct 13, 2006·Current Opinion in Gastroenterology·M Ciarlet, M K Estes
Feb 13, 2008·Scandinavian Journal of Gastroenterology·Anthony G Catto-SmithRuth F Bishop
Dec 31, 2008·Current Opinion in Gastroenterology·Joseph M Hyser, Mary K Estes
Feb 24, 2016·The Journal of General Virology·Kota OkaderaMakoto Sugiyama
Dec 23, 2000·The Journal of General Virology·M van NiekerkH Huismans
Oct 31, 2003·Microbiology and Immunology·Mohammed Ali BorganNobuyuki Minamoto
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Oct 7, 2006·Current Opinion in Gastroenterology·R D Shaw
Mar 15, 2011·Pflügers Archiv : European journal of physiology·Jiraporn OusingsawatKarl Kunzelmann
May 19, 2007·Journal of Medical Microbiology·Irene Trigueiros AraújoJosé Paulo G Leite
Jul 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Neung-Seon SeoMary K Estes
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Jul 2, 2008·Memórias do Instituto Oswaldo Cruz·Talissa de Moraes TavaresDivina das Dôres de Paula Cardoso
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Aug 19, 2017·Emerging Infectious Diseases·Chiara BusiM Beatrice Boniotti
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Jan 7, 2017·Journal of Veterinary Science·Sharad SaurabhYashpal S Malik
Apr 6, 2021·Journal of Medical Virology·Parinaz KhalkhaliHadi Razavi Nikoo

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