Mutations in sticky lead to defective organization of the contractile ring during cytokinesis and are enhanced by Rho and suppressed by Rac

The Journal of Cell Biology
Pier Paolo D'AvinoDavid M Glover

Abstract

The contractile ring is a highly dynamic structure, but how this dynamism is accomplished remains unclear. Here, we report the identification and analysis of a novel Drosophila gene, sticky (sti), essential for cytokinesis in all fly proliferating tissues. sti encodes the Drosophila orthologue of the mammalian Citron kinase. RNA interference-mediated silencing of sti in cultured cells causes them to become multinucleate. Components of the contractile ring and central spindle are recruited normally in such STICKY-depleted cells that nevertheless display asymmetric furrowing and aberrant blebbing. Together with an unusual distribution of F-actin and Anillin, these phenotypes are consistent with defective organization of the contractile ring. sti shows opposite genetic interactions with Rho and Rac genes suggesting that these GTPases antagonistically regulate STICKY functions. Similar genetic evidence indicates that RacGAP50C inhibits Rac during cytokinesis. We discuss that antagonism between Rho and Rac pathways may control contractile ring dynamics during cytokinesis.

References

Apr 1, 1989·Genes & Development·M Gatti, B S Baker
Oct 1, 1995·The Journal of Cell Biology·C M Field, B M Alberts
Dec 18, 1995·FEBS Letters·P MadauleS Narumiya
Aug 23, 1996·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Jan 1, 1997·Current Biology : CB·D N DrechselM Glotzer
May 15, 1997·Nature·D I StruttM Mlodzik
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·M J Rebecchi, S Scarlata
Feb 27, 1999·Current Opinion in Cell Biology·C FieldK Oegema
Mar 8, 2000·The Journal of Cell Biology·S N ProkopenkoH J Bellen
May 10, 2000·Trends in Cell Biology·D N Robinson, J A Spudich
May 18, 2000·Microscopy Research and Technique·P MadauleS Narumiya
Nov 1, 2001·Annual Review of Cell and Developmental Biology·M Glotzer
Mar 29, 2002·Nature·Satoko Hakeda-SuzukiBarry J Dickson
Mar 29, 2002·Nature·Julian NgLiqun Luo
Jul 23, 2002·Molecular Biology of the Cell·Maria Patrizia SommaMaurizio Gatti
Aug 15, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D F R MurisR J A M Michalides
Nov 15, 2002·Journal of Cell Science·Ferdinando Di CuntoLorenzo Silengo
Dec 7, 2002·Journal of Pediatric Hematology/oncology·Yi Gu, David A Williams
Apr 12, 2003·Developmental Cell·Yukinori MinoshimaToshio Kitamura
Jun 13, 2003·Molecular Biology of the Cell·Shigeko YamashiroFumio Matsumura
Nov 21, 1996·Nature·P M BrakefieldS B Carroll
Jul 16, 2003·The Journal of Cell Biology·Hisayoshi YoshizakiMichiyuki Matsuda
Jan 28, 2004·Cell·Keith Burridge, Krister Wennerberg

❮ Previous
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Citations

Feb 22, 2012·Cellular and Molecular Life Sciences : CMLS·Maria Teresa Abreu-BlancoSusan M Parkhurst
May 9, 2012·Cellular and Molecular Life Sciences : CMLS·Sarah M Heissler, Dietmar J Manstein
Jun 7, 2005·Trends in Cell Biology·Fumio Matsumura
Feb 4, 2009·Nature Cell Biology·Pier Paolo D'Avino, David M Glover
Oct 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Bharesh K ChauhanRichard A Lang
May 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zuni I BassiPier Paolo D'Avino
Nov 13, 2013·The Journal of Cell Biology·Nour El AmineGilles R X Hickson
Oct 21, 2005·Molecular Biology of the Cell·Keiju KamijoToru Miki
Oct 5, 2007·Molecular Biology of the Cell·Maria Grazia GiansantiMaurizio Gatti
Aug 19, 2011·Molecular Biology of the Cell·Marta GaiFerdinando Di Cunto
Feb 2, 2012·The Journal of Clinical Investigation·Murielle P SerresArnaud Besson
Mar 5, 2005·Cell Biology Education·Christopher Watters
May 12, 2011·PloS One·João J E RochaCahir J O'Kane
Jul 19, 2012·Annual Review of Cell and Developmental Biology·Rebecca A GreenKaren Oegema
Aug 17, 2005·The Journal of Cell Biology·Ozlem YüceMichael Glotzer
Sep 5, 2012·The Journal of Cell Biology·Tim Davies, Julie C Canman
Sep 5, 2012·The Journal of Cell Biology·Ricardo Nunes BastosFrancis A Barr
Nov 16, 2011·The Journal of Cell Biology·Zuni I BassiPier Paolo D'Avino
Dec 17, 2009·The Journal of Cell Biology·Julie C Canman
Jan 25, 2006·The Journal of Cell Biology·Ulrike GrunebergFrancis A Barr
Jan 24, 2016·Seminars in Cell & Developmental Biology·Pier Paolo D'Avino, Luisa Capalbo
Nov 29, 2011·Trends in Cell Biology·Romain Levayer, Thomas Lecuit
Dec 10, 2014·Developmental Cell·Beata Mierzwa, Daniel W Gerlich
Oct 11, 2012·Cytoskeleton·Shawn N Jordan, Julie C Canman
Aug 14, 2012·Cytoskeleton·Clemens Cabernard
Apr 5, 2014·Genesis : the Journal of Genetics and Development·Michael Sfregola
Feb 23, 2010·IUBMB Life·Stephen L GregoryRobert Saint
Nov 9, 2004·Current Opinion in Cell Biology·Patrick O HumbertHelena E Richardson
Oct 26, 2005·Trends in Cell Biology·Alisa PieknyMichael Glotzer
Oct 6, 2005·Molecular and Cellular Neurosciences·Wandong Zhang, Deanna L Benson

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Methods Mentioned

BETA
GTPases
nucleotide
GTPase
two-hybrid
transgenic
fluorescence resonance
PCR
FCS

Software Mentioned

CIT

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