Mutations in the β-Subunit of the RNA Polymerase Impair the Surface-Associated Motility and Virulence of Acinetobacter baumannii

Infection and Immunity
María Pérez-VarelaJordi Barbé

Abstract

Acinetobacter baumannii is a major cause of antibiotic-resistant nosocomial infections worldwide. In this study, several rifampin-resistant spontaneous mutants obtained from the A. baumannii ATCC 17978 strain that differed in their point mutations in the rpoB gene, encoding the β-subunit of the RNA polymerase, were isolated. All the mutants harboring amino acid substitutions in position 522 or 540 of the RpoB protein were impaired in surface-associated motility and had attenuated virulence in the fertility model of Caenorhabditis elegans The transcriptional profile of these mutants included six downregulated genes encoding proteins homologous to transporters and metabolic enzymes widespread among A. baumannii clinical isolates. The construction of knockout mutants in each of the six downregulated genes revealed a significant reduction in the surface-associated motility and virulence of four of them in the A. baumannii ATCC 17978 strain, as well as in the virulent clinical isolate MAR002. Taken together, our results provide strong evidence of the connection between motility and virulence in this multiresistant nosocomial pathogen.

References

Apr 1, 1994·Antimicrobial Agents and Chemotherapy·L P MillerT M Shinnick
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y N Zhou, D J Jin
Dec 24, 2002·Infection and Immunity·Barbara AlbigerAnn-Beth Jonsson
Mar 20, 2008·PloS One·David VallenetStéphane Cruveiller
May 8, 2008·Nature Reviews. Microbiology·Ken F Jarrell, Mark J McBride
Jul 16, 2008·Clinical Microbiology Reviews·Anton Y PelegDavid L Paterson
Feb 27, 2009·FEMS Microbiology Letters·David A Coil, Jozef Anné
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mitchell T ButlerRasika M Harshey
Nov 11, 2010·BMC Microbiology·Jesús ArandaGermán Bou
Mar 24, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Subramaniapillai KolappanLisa Craig
Apr 27, 2012·FEMS Immunology and Medical Microbiology·Veronika HolaFilip Ruzicka
May 9, 2012·International Journal of Medical Microbiology : IJMM·Evelyn SkiebeGottfried Wilharm
May 10, 2012·FEMS Microbiology Reviews·Michael J McConnellJerónimo Pachón
Jan 15, 2013·Journal of Bacteriology·Matthew D NortonVeronica G Godoy
May 8, 2013·Infection and Immunity·Bart A EijkelkampMelissa H Brown
Oct 15, 2013·Journal of Bacteriology·Jesús ArandaGermán Bou
Sep 4, 2014·Journal of Bacteriology·Sasikiran PasupuletiMichael D Manson
Dec 8, 2014·Journal of Biotechnology·Pietro AlifanoAdelfia Talà
Feb 13, 2015·Molecular Plant-microbe Interactions : MPMI·Tiyakhon ChatnaparatYoufu Zhao
Sep 30, 2015·Antimicrobial Agents and Chemotherapy·Roberta ColicchioPaola Salvatore
Oct 20, 2015·Current Opinion in Microbiology·Barbara I KazmierczakRuchi Jain
Feb 18, 2016·Scientific Reports·So-ichiro NishiyamaIkuro Kawagishi
Jul 3, 2016·Pathogens and Disease·Grace A MaldarelliErik C von Rosenvinge
Oct 8, 2016·Microbial Cell Factories·Mercedes Berlanga, Ricardo Guerrero
Apr 5, 2017·The Journal of Infectious Diseases·Gerald L MurrayAnton Y Peleg

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Citations

Jun 27, 2018·Antimicrobial Agents and Chemotherapy·María Pérez-VarelaJordi Barbé
Dec 19, 2017·Nature Reviews. Microbiology·Christian M HardingMario F Feldman
May 28, 2019·BMC Microbiology·Jiakai SunAnchun Cheng
Mar 1, 2020·BMC Microbiology·Hua ZhouXiaoting Hua
Feb 23, 2021·Microbiological Research·Hing Jian MeaEng Hwa Wong
Nov 20, 2020·Chemical Reviews·Philip A KlenoticEdward W Yu
Aug 13, 2021·Frontiers in Molecular Biosciences·Martina PasquaBianca Colonna

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