Mutations in the coat protein of a begomovirus result in altered transmission by different species of whitefly vectors

Virus Evolution
Li-Long PanShu-Sheng Liu

Abstract

For many crop pathogens including viruses, high genetic variation provides them with potential to adapt to and prevail in a changing environment. Understanding genetic variation in viruses and their significance is a key to elaborate virus epidemiology and evolution. While genetic variation of plant viruses has been documented to impact virus-host interactions, how it affects virus-insect vector interactions remains elusive. Here, we report the impact of mutations in the coat protein of squash leaf curl China virus (SLCCNV), a begomovirus, on the interaction between the virus and its whitefly vectors. We characterized mutations in the coat protein of SLCCNV and found that some residues exhibited higher mutation frequency than the others. We assayed the impact of mutation on infectivity using agroinoculation and found these mutations marginally affect virus infectivity. We further analyze their functions using virus acquisition and transmission trials and found some of mutations resulted in altered transmission of SLCCNV by different species of the whitefly Bemisia tabaci complex. We then identified the key amino acid residue(s) involved by constructing several mutant viruses and found that a single-residue mutation in the coat ...Continue Reading

References

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Datasets Mentioned

BETA
AM260206
MG525551
MG525552
AM260208
KM821540
KC540757
DQ309077
AM286794

Methods Mentioned

BETA
PCR

Software Mentioned

SPSS
DNAMAN

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