Mutations in the hemolytic-phospholipase C operon result in decreased virulence of Pseudomonas aeruginosa PAO1 grown under phosphate-limiting conditions.

Infection and Immunity
R M OstroffM L Vasil

Abstract

The phospholipase C (PLC) operon of Pseudomonas aeruginosa consists of plcS, which encodes a heat-labile secreted hemolysin, and two in-phase, overlapping genes, plcR1 and plcR2, which may encode Pi-regulatory genes. A 2.8-kilobase-pair deletion mutation in this operon was constructed, and a tetracycline resistance (Tcr) cartridge replaced the deleted sequences. A deletion mutant of strain PAO1 was obtained through recombination between the flanking regions of the mutated cloned PLC operon and the homologous chromosomal regions. The deletion of the chromosomal PLC operon and its replacement by the Tcr cartridge was confirmed by Southern hybridization. The deletion strain, PLC SR, is nonhemolytic. However, it retains PLC activity when measured on a synthetic substrate. A second mutant strain, PLC R, contains a deletion in the plcR genes. This mutant is more hemolytic and produces more enzymatic activity than PAO1. The virulence of both of these mutants was compared with that of PAO1 in the mouse burn model of infection. When mice were infected with cultures grown in a high-Pi medium, there was a 10-fold increase in the 50% lethal dose of the mutants compared with PAO1. In contrast, when the inoculum originated from low-Pi cultur...Continue Reading

References

Mar 1, 1979·Microbiological Reviews·B W HollowayA F Morgan
Aug 1, 1979·Infection and Immunity·M W Stinson, C Hayden
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R K TaylorJ J Mekalanos
May 1, 1986·Infection and Immunity·M L VasilC C Grant
Jul 1, 1987·Infection and Immunity·R S BerkD Meyers
Sep 1, 1988·Thrombosis Research·I R CoutinhoE Mammen
Nov 1, 1974·The Journal of Infectious Diseases·P V Liu
May 31, 1974·Science·E D Weinberg
Jan 1, 1981·Cold Spring Harbor Symposia on Quantitative Biology·D E BergT D Tlsty
Nov 1, 1981·Journal of Medical Microbiology·E O IdigbeA C Wardlaw
Sep 1, 1980·Infection and Immunity·M K Johnson, D Boese-Marrazzo
Jun 1, 1961·Journal of Bacteriology·M T ESSELMANN, P V LIU

❮ Previous
Next ❯

Citations

Feb 3, 1998·American Journal of Respiratory Cell and Molecular Biology·J F PittetJ P Wiener-Kronish
Aug 10, 2002·Journal of Bacteriology·Paula J WildermanMichael L Vasil
Aug 11, 2006·The Journal of Biological Chemistry·Richard L FeltsJohn J Tanner
Oct 15, 2010·Acta Crystallographica. Section D, Biological Crystallography·S G VachieriA K Basak
Dec 22, 2005·Infection and Immunity·Lena J HeungMaurizio Del Poeta
Dec 24, 2008·Infection and Immunity·Matthew J WargoDeborah A Hogan
May 13, 2011·American Journal of Respiratory and Critical Care Medicine·Matthew J WargoDeborah A Hogan
May 1, 1993·Journal of Periodontal Research·J D HillmanS S Socransky
Jul 1, 1990·The American Review of Respiratory Disease·L M GrahamK R Stenmark
May 6, 2003·Journal of Bacteriology·Mercedes M MarínFernando Rojo
Jan 5, 2007·The Journal of Biological Chemistry·Nozomu Okino, Makoto Ito
Nov 17, 2012·Microbiology·Audrey DagornMarc G J Feuilloley
Jun 17, 2016·Microbiology and Molecular Biology Reviews : MMBR·Marietta Flores-DíazAntje Flieger
Dec 27, 2013·Nihon saikingaku zasshi. Japanese journal of bacteriology·Yutaka Kida
Nov 10, 2001·Annual Review of Phytopathology·H CaoL G Rahme
Nov 2, 2002·Molecular Microbiology·Martin J StonehouseMichael L Vasil
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Urs A OchsnerMichael L Vasil

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.