PMID: 9449271Feb 4, 1998Paper

Mutations in topoisomerase IV and DNA gyrase of Staphylococcus aureus: novel pleiotropic effects on quinolone and coumarin activity

Antimicrobial Agents and Chemotherapy
B Fournier, D C Hooper

Abstract

Previous studies have shown that topoisomerase IV and DNA gyrase interact with quinolones and coumarins in different ways. The MICs of coumarins (novobiocin and coumermycin) for MT5, a Staphylococcus aureus nov mutant, are higher than those for wild-type strains. Sequencing the gyrB gene encoding one subunit of the DNA gyrase revealed the presence of a double mutation likely to be responsible for this resistance: at codon 102 (Ile to Ser) and at codon 144 (Arg to Ile). For single-step flqA mutant MT5224c9, previously selected on ciprofloxacin, the fluoroquinolone MIC was higher and the coumarin MIC was lower than those for its parent, MT5. Sequencing the grlB and grlA genes of topoisomerase IV of MT5224c9 showed a single Asn-470-to-Asp mutation in GrlB. Genetic outcrosses by transformation with chromosomal DNA and introduction of plasmids carrying either the wild-type or the mutated grlB gene indicated that this mutation causes both increased MICs of fluoroquinolones and decreased MICs of coumarins and that the mutant grlB allele is codominant for both phenotypes with multicopy alleles. Integration of these plasmids into the chromosome confirmed the codominance of fluoroquinolone resistance, but grlB+ appeared dominant over grl...Continue Reading

References

Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M GellertJ Tomizawa
Oct 1, 1975·Journal of Bacteriology·P A Pattee, D S Neveln
Sep 1, 1991·Journal of Bacteriology·M TrucksisD C Hooper
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·I del CastilloF Moreno
Aug 1, 1991·Antimicrobial Agents and Chemotherapy·H YoshidaS Nakamura
Dec 1, 1991·Antimicrobial Agents and Chemotherapy·N NakanishiS Mitsuhashi
Jan 1, 1991·Journal of Bacteriology·M L Holmes, M L Dyall-Smith
Oct 1, 1985·Journal of Bacteriology·C Y Lee, J J Iandolo
Jan 26, 1987·Nucleic Acids Research·T AdachiK Mizuuchi
Mar 1, 1980·Journal of Bacteriology·A Sugino, K F Bott
Sep 1, 1994·Antimicrobial Agents and Chemotherapy·H ItoS Nakamura
May 1, 1994·Molecular Microbiology·E J Gilbert, A Maxwell
Aug 1, 1993·Molecular Microbiology·A Maxwell
Jun 1, 1993·Journal of Bacteriology·S M Brockbank, P T Barth
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·A B KhodurskyN R Cozzarelli
Jan 18, 1996·Nature·J M BergerJ C Wang
Apr 1, 1996·Antimicrobial Agents and Chemotherapy·M StiegerH Gmünder
Oct 1, 1996·Antimicrobial Agents and Chemotherapy·X S PanL M Fisher
Dec 1, 1996·Antimicrobial Agents and Chemotherapy·F BlancheJ Crouzet

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Citations

Mar 16, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D C Hooper
Jan 30, 2013·Antimicrobial Agents and Chemotherapy·Yuko YamaguchiTakashi Deguchi
Nov 18, 2003·Journal of Bacteriology·Dilek Ince, David C Hooper
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Patricia Komp LindgrenDiarmaid Hughes
Feb 27, 2008·Journal of Applied Microbiology·A SutandarL Y Hsu
Nov 26, 2008·Chemistry : a European Journal·Alexander V LyginArmin de Meijere
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Michael F MinnickD Scott Samuels
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Dilek InceDavid C Hooper
Mar 26, 2003·Antimicrobial Agents and Chemotherapy·Dilek InceDavid C Hooper
Jul 25, 2003·Antimicrobial Agents and Chemotherapy·David R MacingaTimothy W Morris
Feb 17, 2005·Journal of Medical Microbiology·Ashraf M AhmedTadashi Shimamoto
Apr 25, 2001·Applied and Environmental Microbiology·T B StantonS B Humphrey
Sep 29, 2006·Journal of Medical Microbiology·Yoshihiro OyamadaJun-ichi Yamagishi
Nov 25, 2004·Antimicrobial Agents and Chemotherapy·Jeffrey J CampionMartin E Evans
Aug 30, 2005·Antimicrobial Agents and Chemotherapy·Mika Fujimoto-NakamuraJun-Ichi Yamagishi
Feb 7, 2018·International Journal of Molecular Sciences·Sebastian Franco-UlloaMarco De Vivo
Oct 31, 1998·Antimicrobial Agents and Chemotherapy·X S Pan, L M Fisher
Oct 31, 1998·Antimicrobial Agents and Chemotherapy·M TanakaK Sato
Aug 4, 1998·Antimicrobial Agents and Chemotherapy·B Fournier, D C Hooper

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