PMID: 8950190Nov 11, 1996Paper

Mutations in yeast ribosomal proteins S28 and S4 affect the accuracy of translation and alter the sensitivity of the ribosomes to paromomycin

Biochimica Et Biophysica Acta
D SynetosL E Alksne

Abstract

Ribosomal proteins S12, S5 and S4 of Escherichia coli are essential for the control of translational accuracy. Their yeast equivalents, i.e., S28, S4 and S13, have also been implicated in this process. Using a poly(U)-dependent cell-free translation system, we determined the accuracy of translation and the sensitivity to antibiotic paromomycin of yeast ribosomes carrying mutant ribosomal proteins S28 and/or S4. Our results confirm by quantitative biochemical methods previous genetic data showing that proteins S28 and S4 are involved in the decoding activity of the ribosome and interact to control translational accuracy. We find that the suppressor mutation SUP44 in yeast S4, decreased the accuracy of translation. To examine the effect of mutant S28, we disrupted RPS28B and introduced in RPS28A the same substitutions that cause hyperaccurate translation or antibiotic resistance in bacteria. Three of these substitutions (Lys-62-->Asn, Thr or Gln) similarly increased translational accuracy in vitro or antibiotic resistance. In the presence of the SUP44 mutation, these substitutions partially reversed the decrease of translational accuracy caused by SUP44. However, the Lys-62-->Arg substitution decreased translational accuracy and ...Continue Reading

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Citations

Jun 5, 2007·Genes & Development·Susanne Röther, Katja Strässer
Oct 1, 2004·Molecular and Cellular Biology·Magdalena Rakwalska, Sabine Rospert
Jun 2, 2005·Reviews of Physiology, Biochemistry and Pharmacology·S RospertY Dubaquié
Jan 2, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xiaobang HuChangliang Zhu
Dec 9, 2014·Prion·Anton A NizhnikovIrina L Derkatch
May 1, 2015·Nucleic Acids Research·Petra BeznoskováLeoš Shivaya Valášek
Oct 26, 2005·Molecular Cell·Sébastien Ferreira-CercaPhilipp Milkereit
Mar 17, 2010·Biochimica Et Biophysica Acta·Kristin PeiskerSabine Rospert
Nov 13, 2014·Nucleic Acids Research·Sharmishtha MusalgaonkarJonathan D Dinman
May 10, 2008·The EMBO Journal·Andrey V PisarevTatyana V Pestova
May 1, 2019·Cellular and Molecular Life Sciences : CMLS·Rakesh SrivastavaSeong Hoon Ahn
Nov 16, 2002·The Journal of Biological Chemistry·John DresiosDennis Synetos

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