PMID: 11336987May 5, 2001Paper

Mutations induced by 5-formyl-2'-deoxyuridine in Escherichia coli include base substitutions that can arise from mispairs of 5-formyluracil with guanine, cytosine and thymine

Mutation Research
H AnensenS Bjelland

Abstract

5-Formyluracil (5-foU) is a major oxidation product of thymine formed in yields comparable to that of 8-oxoguanine in DNA by ionizing radiation. Whereas the mutagenic effects of 8-oxoguanine are well understood, the investigation of the biological implications of 5-foU has so far been limited. Here we demonstrate that 5-formyl-2'-deoxyuridine (5-fodUrd) supplied to the growth medium of Escherichia coli induces several base substitutions at different frequencies at position 461 in the lacZ gene in the following order: A.T-->G.C>G.C-->A.T>G.C-->T.A>A.T-->T.A>A.T-->C.G. No induction of G.C-->C.G transversions was observed. It is inferred that 5-fodUrd will be incorporated into the DNA during cell growth, forming mispairs with guanine, cytosine and thymine during replication. It, thus, appears that cell growth in the presence of 5-fodUrd may represent a good model for elucidating the cellular effects of 5-foU residues in DNA.

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Citations

Nov 26, 2003·Mutation Research·Svein Bjelland, Erling Seeberg
Jun 27, 2001·Mutation Research·S BjellandE Seeberg
Nov 13, 2003·Bioorganic & Medicinal Chemistry Letters·Toru SugiyamaReiko Kuroda
Nov 24, 2001·Mutation Research·Hiroyuki KamiyaHiroshi Kasai
Jan 18, 2003·DNA Repair·Pingfang LiuLawrence C Sowers
Aug 7, 2013·Journal of Medicinal Chemistry·Laurent F BonnacSteven E Patterson
Sep 26, 2007·Nature Clinical Practice. Endocrinology & Metabolism·Knut KrohnRalf Paschke
Jul 15, 2006·Nucleosides, Nucleotides & Nucleic Acids·Daisuke IrieShunji Izuta
Jul 26, 2002·Nucleic Acids Research·Izumi MiyabeShuji Yonei
Dec 19, 2002·American Journal of Human Genetics·M Thomas P GilbertAlan Cooper
Jan 12, 2017·Chemical Communications : Chem Comm·F KawasakiS Balasubramanian
Mar 6, 2010·Organic & Biomolecular Chemistry·Sriram Kanvah, Gary B Schuster
Mar 12, 2020·Frontiers in Microbiology·Kristin GrøsvikSvein Bjelland
Apr 15, 2009·International Journal of Radiation Biology·Ingeborg KnaevelsrudSvein Bjelland
Dec 17, 2008·Current Protocols in Nucleic Acid Chemistry·Kousuke SatoAkira Matsuda
Feb 21, 2019·Chemical Research in Toxicology·Hiroshi KasaiKazuaki Kawai

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