PMID: 2120584Oct 1, 1990Paper

Mutations induced by benzo[a]pyrene diolepoxide at the hprt locus in human T-lymphocytes in vitro

Mutation Research
B Andersson, B Lambert

Abstract

Human T-lymphocytes have been treated with benzo[a]pyrene diolepoxide (BPDE) in vitro and T-cell clones mutated in the hprt gene have been isolated. The mutant frequencies in BPDE-treated T-cell cultures were on average 24-fold higher than those of untreated cultures. Thus, BPDE is a potent inducer of gene mutation in this system. In order to examine which types of mutations are induced by BPDE in human cells, 41 spontaneous and 44 BPDE-induced mutant clones have been characterized using the Southern blot technique. In addition, rearrangements of the T-cell-receptor beta and gamma loci have been used to determine the proportion of isolated clones that are unique, and thus likely to represent independent mutational events. Out of 23 independent spontaneous mutants 4 had large hprt alterations that could be detected on Southern blots. Two of these alterations, deletions of exons 2-6, have been confirmed using PCR of hprt cDNA and direct sequencing of the PCR product. All 33 independent BPDE-induced mutants had normal hprt restriction patterns which indicates that BPDE is mainly a point mutagen in this system.

References

Jan 1, 1977·Mutation Research·P Brookes
Apr 1, 1990·Genomics·A EdwardsW Ansorge
Jan 1, 1985·Annual Review of Genetics·J T Stout, C T Caskey
Dec 1, 1985·Mutation Research·A A MorleyR S Seshadri
Oct 25, 1985·Nucleic Acids Research·K C Reed, D A Mann
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D L BusbeeP W Rankin
Apr 1, 1984·Mutation Research·S ChakrabartiM Seidman
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R J AlbertiniW R Borcherding
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E EisenstadtJ H Miller

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Citations

Dec 1, 1995·Toxicology Letters·B LambertS M Hou
Dec 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·N ZanesiL Celotti
Dec 31, 1997·Mutation Research·A AidooD A Casciano
Jan 1, 1992·Environmental and Molecular Mutagenesis·B AnderssonB Lambert
Jan 1, 1995·Environmental and Molecular Mutagenesis·S M HouC E Vaca

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