PMID: 275843Jun 1, 1978

Mutations of bacteriophage T7 that affect initiation of synthesis of the gene 0.3 protein

Proceedings of the National Academy of Sciences of the United States of America
J J DunnF W Studier

Abstract

Two different mutations that greatly diminish the rate of synthesis of the gene 0.3 protein of bacteriophage T7 have been characterized. One is in the initiator triplet for the 0.3 protein, changing it from AUG to ACG. This mutation was found to have little effect on binding of ribosomes to the 0.3 mRNA in vitro, although 0.3 protein synthesis was greatly depressed in vitro as well as in vivo. A suppressor mutation that partially restores the wild-type rate of synthesis was found to lie within the 0.3 RNA but not close to the mutant ACG (more than 64 nucleotides away). The second mutation is a G-to-A transition located 11 bases to the 5' side of the initiator AUG. This change eliminates a possible five-base pairing with a sequence near the 3' end of 16S ribosomal RNA, an interaction previous workers have proposed to be important for initiation of protein synthesis. This mutation causes the site of ribosome binding to shift about 15 bases to the 3' side, centering on an internal AUG, but this new site has only a poor potential interaction with 16S RNA. A suppressor mutation that restores the rate of 0.3 protein synthesis to essentially wild-type levels and also restores wild-type ribosome-binding behavior was found to lie adjace...Continue Reading

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Related Concepts

Sense Codon
Coliphages
Genes, Viral
Peptide Chain Initiation, Translational
Polyribosomes
Ribosomes
Poly(A) Tail
15S RNA
Suppressor Mutation

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