Mutations of Helicobacter pylori RdxA are mainly related to the phylogenetic origin of the strain and not to metronidazole resistance.

The Journal of Antimicrobial Chemotherapy
Shuzhen ZhangEng Guan Chua

Abstract

Drug resistance of Helicobacter pylori is a major clinical problem worldwide. The objective of the present study was to investigate the prevalence of antibiotic-resistant H. pylori in the city of Shenzhen in China, as well as to identify the genetic mutations specifically associated with drug resistance rather than unrelated phylogenetic signals. Antibiotic susceptibility testing was performed on 238 clinical strains successfully isolated from H. pylori-positive dyspeptic patients who underwent gastroscopy at the Department of Gastroenterology in Shenzhen People's Second Hospital. Following WGS of all strains using Illumina technology, mutation and phylogenetic analyses were performed. The resistance rates were 84.9%, 35.3%, 25.2% and 2.1% for metronidazole, clarithromycin, ciprofloxacin and rifampicin, respectively. An A2143G conversion in the 23S rRNA gene was the primary mutation observed in clarithromycin-resistant strains, whilst N87K/I and D91G/N/Y in GyrA were detected in ciprofloxacin-resistant strains. In RdxA, our results demonstrated that only R16H/C and M21A are significant contributors to metronidazole resistance; there were 15 other sites, but these are phylogenetically related and thus unrelated to metronidazole ...Continue Reading

References

Nov 4, 2000·The Journal of Antimicrobial Chemotherapy·D H KwonJ Versalovic
Apr 10, 2002·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M HeepW Wehrl
Nov 9, 2011·Antimicrobial Agents and Chemotherapy·Magali GarciaChristophe Burucoa
Nov 2, 2016·Gut·P MalfertheinerUNKNOWN European Helicobacter and Microbiota Study Group and Consensus panel
Feb 9, 2017·Antimicrobial Agents and Chemotherapy·Derrick ChenRobin Patel
Nov 24, 2017·Helicobacter·Constantin HaysJosette Raymond
Mar 8, 2018·Helicobacter·Wen Zhong LiuUNKNOWN Chinese Society of Gastroenterology, Chinese Study Group on Helicobacter pylori and Peptic Ulcer
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Aining ChuYuehua Gong

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