PMID: 2109098May 1, 1990Paper

Mutations of RNA and protein sequences involved in human immunodeficiency virus type 1 packaging result in production of noninfectious virus

Journal of Virology
A Aldovini, R A Young

Abstract

To identify RNA and protein sequences involved in packaging of human immunodeficiency virus type 1 (HIV-1), various mutations were introduced into the viral genome. Portions of the human immunodeficiency virus type 1 genome between the first splice donor site and the gag initiation codon were deleted to investigate the RNA packaging site (psi). Point mutations that alter cysteine residues in one or both zinc finger motifs of p7, a cleavage product of the gag precursor, were created to study the role of the gag zinc fingers in packaging. The psi site mutants and the gag mutants exhibited similar phenotypes. Cells transfected with the mutant genomes, while expressing normal levels of human immunodeficiency virus type 1 RNA and proteins, produced viral particles that were normal in protein content but lacked detectable viral RNA. These mutant virions were unable to productively infect cells. The combination of human immunodeficiency virus type 1 packaging mutations should minimize fortuitous assembly of infectious virus and may provide a means to produce noninfectious particles for candidate vaccines.

References

Jul 18, 1985·Nature·A G FisherF Wong-Staal
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R C DesrosiersM D Daniel
Jan 24, 1985·Nature·L RatnerK Baumeister
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F di Marzo VeroneseR C Gallo
Jan 15, 1988·Cell·R M Evans, S M Hollenberg
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R J GorelickA Rein
Jun 7, 1985·Science·M D DanielR C Desrosiers
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Jan 1, 1987·AIDS Research and Human Retroviruses·F D VeroneseM G Sarngadharan
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
May 21, 1982·Science·H E Varmus
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S Watanabe, H M Temin

❮ Previous
Next ❯

Citations

Feb 1, 1993·Protein Science : a Publication of the Protein Society·F Dib-HajjD P Giedroc
Mar 1, 1997·Nucleic Acids Research·J A BerglundM Rosbash
Nov 1, 1996·International Journal of Peptide and Protein Research·H JiM Shao
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·P CorbeauF Wong-Staal
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·A Y Louie, T J Meade
Oct 9, 2001·Human Gene Therapy·M KumarR E Sutton
Jul 9, 2010·Journal of Virology·Sarra E KeeneAlice Telesnitsky
Mar 12, 2011·Nucleic Acids Research·Ben BerkhoutTruus E M Abbink
May 18, 2012·Journal of Virology·Sarra E Keene, Alice Telesnitsky
Apr 5, 2013·PLoS Pathogens·Olga A NikolaitchikWei-Shau Hu
Jun 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yantao YangAntony K Chen
Sep 13, 2020·Viruses·Jennifer L Elliott, Sebla B Kutluay
Mar 21, 2000·Medicinal Research Reviews·H JonckheereE De Clercq
Apr 10, 1999·The Journal of Biological Chemistry·S DruillennecB P Roques

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.