Mutations of RNA polymerase II activate key genes of the nucleoside triphosphate biosynthetic pathways.

The EMBO Journal
Marta KwapiszFrançois Lacroute

Abstract

The yeast URA2 gene, encoding the rate-limiting enzyme of UTP biosynthesis, is transcriptionally activated by UTP shortage. In contrast to other genes of the UTP pathway, this activation is not governed by the Ppr1 activator. Moreover, it is not due to an increased recruitment of RNA polymerase II at the URA2 promoter, but to its much more effective progression beyond the URA2 mRNA start site(s). Regulatory mutants constitutively expressing URA2 resulted from cis-acting deletions upstream of the transcription initiator region, or from amino-acid replacements altering the RNA polymerase II Switch 1 loop domain, such as rpb1-L1397S. These two mutation classes allowed RNA polymerase to progress downstream of the URA2 mRNA start site(s). rpb1-L1397S had similar effects on IMD2 (IMP dehydrogenase) and URA8 (CTP synthase), and thus specifically activated the rate-limiting steps of UTP, GTP and CTP biosynthesis. These data suggest that the Switch 1 loop of RNA polymerase II, located at the downstream end of the transcription bubble, may operate as a specific sensor of the nucleoside triphosphates available for transcription.

References

Jul 1, 1992·Current Genetics·F Exinger, F Lacroute
Jul 1, 1989·Molecular and Cellular Biology·N A Woychik, R A Young
Sep 1, 1987·Molecular & General Genetics : MGG·S PotierF Lacroute
Jul 1, 1965·Journal of General Microbiology·F LacrouteJ M Wiame
Jan 1, 1981·Molecular & General Genetics : MGG·R Losson, F Lacroute
Feb 1, 1994·Molecular & General Genetics : MGG·O Ozier-KalogeropoulosF Lacroute
Dec 22, 1998·Molecular and Cellular Biology·P J Flynn, R J Reece
Sep 13, 2003·The EMBO Journal·Olivier HarismendyOlivier Lefebvre
May 21, 2005·Nucleic Acids Research·Zhihong Zhang, Fred S Dietrich
Oct 26, 2005·Trends in Biochemical Sciences·Robert Landick
Feb 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Carrie Anne Davis, Manuel Ares
Mar 2, 2006·Molecular Cell·John T ArigoJeffry L Corden
Jun 20, 2006·Molecular and Cellular Biology·Julie SoutourinaMichel Werner
Dec 13, 2006·Molecular Cell·Eric J SteinmetzDavid A Brow
Dec 21, 2006·Nucleic Acids Research·Cécile ZarosFrancisco Navarro
Jan 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kara JuneauRonald W Davis
Feb 14, 2007·Molecular and Cellular Biology·Katarzyna A KopcewiczDaniel Reines
Jun 22, 2007·Nature·Dmitry G VassylyevIrina Artsimovitch
Feb 1, 2008·Nature·Akira HirataKatsuhiko S Murakami
Apr 23, 2008·Molecular and Cellular Biology·M Harley JenksDaniel Reines
Jul 17, 2008·Genes & Development·Yad Ghavi-HelmJulie Soutourina

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Citations

Apr 29, 2010·Current Genetics·Ma Carmen García-LópezFrancisco Navarro
Nov 10, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Benjamin R HarrisonJocelyn E Krebs
Aug 22, 2009·PLoS Genetics·Vicent PelechanoSebastián Chávez
Mar 16, 2012·Genetics·Per O Ljungdahl, Bertrand Daignan-Fornier
May 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Henrik SpåhrRoger D Kornberg
Oct 11, 2008·EMBO Reports·Jeffry L Corden
Jul 21, 2011·Biochimie·Mathieu TisseurAntonin Morillon
Dec 21, 2011·Biochimica Et Biophysica Acta·José E Pérez-OrtínSebastián Chávez
Dec 8, 2016·Nucleic Acids Research·Miguel GaravísOlga Calvo
Aug 31, 2017·Transcription·Olga Calvo
Oct 22, 2018·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Ewa LeśniewskaMagdalena Boguta

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Methods Mentioned

BETA
ChIP
PCR
ChIP-chip
immunoprecipitation

Software Mentioned

Applied Biosytems , System SDS

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