PMID: 9525672Apr 3, 1998Paper

Mutations of the human immunodeficiency virus type 1 p6Gag domain result in reduced retention of Pol proteins during virus assembly

Journal of Virology
X F YuM Dettenhofer

Abstract

One of the crucial steps in the assembly of the human immunodeficiency virus type 1 (HIV-1) and other retroviruses is the incorporation and retention of all the key viral enzymes in released virions. The viral enzymes protease, reverse transcriptase, and integrase of HIV-1 are initially synthesized as Gag-Pol fusion polyproteins. It has been shown that the incorporation of Gag-Pol polyproteins during virus assembly requires the Gag domains that are shared by the Gag and Gag-Pol precursors. We now report that truncation of the C-terminal p6 domain of HIV-1 Gag, which is present in the Gag precursor but not in the Gag-Pol precursor, drastically reduced the amount of Pol proteins in the mutant virions. Mutations in the lentivirus conserved motif P(T/S)APP in p6 also drastically reduced the amount of Pol proteins in mutant virions. The steady-state levels of Gag-Pol precursors and cleaved Pol proteins in the transfected cells were not affected by mutations in p6. The incorporation of unprocessed Gag-Pol precursors into p6 mutant virions was detected when the viral protease was mutated, suggesting that the interactions among mutant Gag molecules and Gag-Pol precursors were not significantly affected. These results suggest that the p...Continue Reading

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Citations

Apr 23, 2002·Journal of Virology·Eric O Freed
May 25, 2004·The Journal of Biological Chemistry·Bénédicte HemonnotLaurence Briant
Aug 30, 2008·AIDS Research and Human Retroviruses·Akinyemi I OjesinaPhyllis J Kanki
Oct 30, 2015·Journal of Virology·Angelica N MartinsEric O Freed
Oct 31, 2003·AIDS Research and Human Retroviruses·N WhitehurstA Gamarnik
Mar 30, 2005·AIDS Research and Human Retroviruses·Robert C ColgroveSeth L Welles
Nov 17, 2009·FEBS Letters·Lawrence KleimanKarin Musier-Forsyth
Oct 29, 2011·Cellular and Molecular Life Sciences : CMLS·Bin XueVladimir N Uversky
Oct 20, 2005·The Journal of Biological Chemistry·Torgils FossenUlrich Schubert
Dec 9, 2004·AIDS Research and Human Retroviruses·Natalia MarloweUNKNOWN Adult AIDS Clinical Trials Groups
Oct 1, 2020·Therapeutic Advances in Infectious Disease·Linh Vu Phuong DangLinus Olson
Oct 20, 2009·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Milos OpravilUNKNOWN Swiss HIV Cohort Study
Oct 24, 2020·Viruses·Kevin M RoseFadila Bouamr

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