Mutations that alter a conserved element upstream of the potato virus X triple block and coat protein genes affect subgenomic RNA accumulation

Virology
K H Kim, Cynthia Hemenway

Abstract

The putative subgenomic RNA (sgRNA) promoter regions upstream of the potato virus X (PVX) triple block and coat protein (CP) genes contain sequences common to other potexviruses. The importance of these sequences to PVX sgRNA accumulation was determined by inoculation of Nicotiana tabacum NT1 cell suspension protoplasts with transcripts derived from wild-type and modified PVX cDNA clones. Analyses of RNA accumulation by S1 nuclease digestion and primer extension indicated that a conserved octanucleotide sequence element and the spacing between this element and the start-site for sgRNA synthesis are critical for accumulation of the two major sgRNA species. The impact of mutations on CP sgRNA levels was also reflected in the accumulation of CP. In contrast, genomic minus- and plus-strand RNA accumulation were not significantly affected by mutations in these regions. Studies involving inoculation of tobacco plants with the modified transcripts suggested that the conserved octanucleotide element functions in sgRNA accumulation and some other aspect of the infection process.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 1, 1991·The Journal of General Virology·J B BancroftG A Mackie
Sep 1, 1990·The Journal of General Virology·T L SitM G AbouHaidar
Jan 1, 1990·Annual Review of Microbiology·M M Lai
Feb 1, 1989·The Journal of General Virology·D ZuidemaJ F Bol
Sep 1, 1989·The Journal of General Virology·T L SitS Holy
Nov 21, 1988·FEBS Letters·K G SkryabinJ G Atabekov
Jan 11, 1988·Nucleic Acids Research·R L ForsterR C Gardner
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Jun 1, 1995·The Plant Journal : for Cell and Molecular Biology·D C BaulcombeS Santa Cruz
May 1, 1995·The Journal of General Virology·L A CalvertR J Shepherd
Sep 1, 1994·The Journal of General Virology·N S LinY H Hsu
Feb 1, 1994·The Journal of General Virology·A G Solovyev Morozov SYu
Mar 1, 1994·The Plant Cell·E P FontesL Hanley-Bowdoin
Jan 1, 1993·Journal of Virology·M Price
Jul 1, 1996·Journal of Virology·S V Doronin, C Hemenway

❮ Previous
Next ❯

Citations

Mar 28, 2012·Molecules and Cells·Sang-Yun ChoKook-Hyung Kim
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·R W SiegelC C Kao
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R W SiegelC C Kao
Dec 7, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Fabien CôtéDenis Leclerc
Dec 5, 2015·Frontiers in Bioengineering and Biotechnology·Christina DickmeisUlrich Commandeur
Mar 1, 2003·Molecular Plant Pathology·Jeffrey S BattenCynthia Hemenway
Apr 25, 2013·PloS One·Valentin V MakarovEugeny N Dobrov
Nov 8, 2015·Biochemistry. Biokhimii︠a︡·E V PutlyaevJ G Atabekov
Sep 8, 2001·The Journal of Biological Chemistry·I R ChoiK A White
Nov 21, 2001·The Journal of Biological Chemistry·Il-Ryong Choi, K Andrew White
Apr 20, 2018·Frontiers in Microbiology·Tamari Chkuaseli, K Andrew White
Apr 30, 2017·Frontiers in Microbiology·I-Hsuan ChenChing-Hsiu Tsai
Mar 7, 2019·Frontiers in Plant Science·Juliane RöderUlrich Commandeur

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.