Mutations that alter transcriptional activation but not DNA binding in the zinc finger of yeast activator HAPI

Nature
K S Kim, L Guarente

Abstract

Transcription of eukaryotic genes requires an interaction between transcription factors that bind to the TATA box region, and transcriptional activators that bind to upstream activating sequences (UASs) or enhancers. Several yeast upstream transcriptional activators, such as GCN4, GAL4 and HAP1, seem to contain separate domains for binding to DNA and activating transcription. The expression of the cytochrome genes CYC1 and CYC7 is controlled by HAP1, which binds to dissimilar DNA sequences in UAS1 of CYC1 and the UAS of CYC7. HAP1 has a zinc-finger DNA-binding domain between amino-acid residues 1 and 148, and a highly acidic C-terminal activation domain between residues 1,308 and 1,483 (ref. 10). A mutant allele of the HAP1 gene, HAP1-18, leads to a change in Ser 63 to Arg 63, immediately adjacent to the zinc finger in the DNA-binding domain. The HAP1-18 mutation specifically abolishes the ability of the protein to bind to UAS1, but greatly increases the ability of the protein to activate transcription of CYC7. We now report that this increase in activation is mediated solely by the CYC7 UAS and the HAP1-18 protein, and also, that it is not caused by an altered binding affinity of the protein for the CYC7 UAS. Furthermore, even...Continue Reading

References

Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C J Brandl, K Struhl
Jun 5, 1987·Science·T ManiatisJ A Fischer
Jan 1, 1987·Annual Review of Genetics·L Guarente
Feb 1, 1983·Cell·A HochschildM Ptashne
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L GuarenteP Gifford

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Citations

May 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C A Stanway
Jul 28, 1995·Molecular & General Genetics : MGG·M L Haldi, L Guarente
Jan 1, 1994·Progress in Biophysics and Molecular Biology·V J Morris
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K S KimL Guarente
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S Oliviero, K Struhl
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S K ThukralE T Young
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L ZhangL Guarente
Nov 1, 1991·Trends in Biochemical Sciences·J N Miner, K R Yamamoto
Sep 1, 1990·Molecular and Cellular Biology·C BuchmanM Karin
Oct 1, 1991·Molecular and Cellular Biology·J C Schneider, L Guarente
Oct 1, 1992·Genes & Development·B Turcotte, L Guarente
Aug 1, 1991·Genes & Development·H WeintraubS J Tapscott

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