PMID: 7017732Apr 1, 1981Paper

Mutator phenotypes in mammalian cell mutants with distinct biochemical defects and abnormal deoxyribonucleoside triphosphate pools

Proceedings of the National Academy of Sciences of the United States of America
G WeinbergD W Martin

Abstract

Recent studies of in vitro DNA synthesis have shown that fidelity of replication is influenced by the relative concentrations of deoxyribonucleoside triphosphates (dNTPs). Several investigators have used reconstituted prokaryotic replication systems to copy defined natural templates and have shown that specific incorporation errors can be induced by an appropriate bias of the precursor pools. The recent demonstration of mutator phenotypes among mutant Chinese hamster ovary cell lines with altered intracellular dNTP pools has allowed extension of the in vitro observations to eukaryotic replication and repair mechanisms. We describe here three mutant murine T-lymphosarcoma cell lines with altered dNTP pools and increased rates of spontaneous mutation to dexamethasone resistance and 6-thioguanine resistance. Unlike previously described mammalian cells with mutator phenotypes, these three lines have demonstrable defects in known structural gene products. Two of these cell lines are heterozygous for mutations affecting the M1 subunit of ribonucleoside diphosphate reductase; the other mutant is deficient in deoxycytidylate deaminase. In each cell line these mutations result in deranged endogenous dNTP pools and increased rates of spo...Continue Reading

References

May 1, 1976·Cancer Letters·W I Schaeffer, K Friend
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B UllmanD V Santi
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A R Fersht
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·U Friedrich, P Coffino
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M MeuthM Trudel
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L A Weymouth, L A Loeb
Jan 1, 1979·Annual Review of Biochemistry·L Thelander, P Reichard
Aug 10, 1978·Nature·M O Bradley, N A Sharkey
Sep 1, 1976·Mutation Research·P M Aebersold
Aug 1, 1974·Cell·C H Sibley, G M Tomkins
Jan 1, 1973·Mutation Research·R L Capizzi, J W Jameson
Apr 1, 1970·Experimental Cell Research·K Horibata, A W Harris
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G Prem veer Reddy, A B Pardee

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Citations

Jan 1, 1985·Cancer Metastasis Reviews·V LingR P Hill
Jun 1, 1987·Molecular & General Genetics : MGG·S AizawaG M Martin
Jan 1, 1986·Current Genetics·E M McIntoshR H Haynes
Jan 1, 1982·Molecular & General Genetics : MGG·L H Johnston, A P Thomas
Sep 1, 1990·Somatic Cell and Molecular Genetics·M ItoD Ayusawa
Mar 1, 1988·Somatic Cell and Molecular Genetics·A Kelsall, M Meuth
Jan 1, 1989·Somatic Cell and Molecular Genetics·I C LiE H Chu
May 1, 1985·Somatic Cell and Molecular Genetics·S AizawaG M Martin
Jul 1, 1985·Somatic Cell and Molecular Genetics·K FukuchiY Okada
Sep 1, 1985·Somatic Cell and Molecular Genetics·G L WeinbergD W Martin
May 26, 2007·Journal of Biomedical Science·Chun-Mei Hu, Zee-Fen Chang
Oct 1, 1983·Mutation Research·I C LiE H Chu
Jul 1, 1988·Mutation Research·R H Haynes, B A Kunz
Jul 1, 1988·Mutation Research·B A Kunz
Oct 1, 1982·Mechanisms of Ageing and Development·J E Dick, J A Wright
Oct 14, 1985·Mechanisms of Ageing and Development·J E Dick, J A Wright
Jan 1, 1984·Pharmacology & Therapeutics·L M Nutter, Y C Cheng
Jul 1, 1985·Mutation Research·N J Sargentini, K C Smith
Feb 6, 2002·Mutation Research·Stella A Martomo, Christopher K Mathews

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