MutMapPlus identified novel mutant alleles of a rice starch branching enzyme IIb gene for fine-tuning of cooked rice texture

Plant Biotechnology Journal
Masaru NakataHiromoto Yamakawa

Abstract

Physicochemical properties of storage starch largely determine rice grain quality and food characteristics. Therefore, modification of starch property is effective to fine-tune cooked rice textures. To obtain new resources with modified starch property as breeding materials, we screened a mutant population of a japonica cultivar Nipponbare and found two independent mutant lines, altered gelatinization (age)1 and age2, with moderate changes in starch gelatinization property. A combination of conventional genetic analyses and the latest mapping method, MutMapPlus, revealed that both of these lines harbour novel independent mutant alleles of starch branching enzyme IIb (BEIIb) gene. In age1, amino acid substitution of Met-723 to Lys completely abolished BEIIb enzyme activity without significant reduction in its protein level. A transposon insertion in an intron of BEIIb gene reduced BEIIb protein level and activity in age2. Production of a series of the mutant lines by combining age alleles and indica-type starch synthase IIa allele established stepwise alteration of the physicochemical properties of starch including apparent amylose content, thermal property, digestibility by α-amylase and branched structures of amylopectin. Cons...Continue Reading

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Citations

Nov 22, 2018·Bioscience, Biotechnology, and Biochemistry·Ken IijimaKatsumi Takano
Oct 1, 2020·Plant Direct·Iny Elizebeth MathewPinky Agarwal
Oct 20, 2018·Frontiers in Plant Science·Gopal MisraNese Sreenivasulu
Nov 30, 2019·Frontiers in Plant Science·Vívian Ebeling VianaAntonio Costa de Oliveira
Jan 8, 2021·Journal of Plant Physiology·Mahendar ThudiRajeev K Varshney
Nov 4, 2020·Journal of Agricultural and Food Chemistry·Changquan ZhangQiaoquan Liu
Sep 25, 2021·Journal of the Science of Food and Agriculture·Xuteng ZhangQiantao Jiang

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Methods Mentioned

BETA
phosphotransferase
transgenic
differential scanning calorimetry

Software Mentioned

MutMapPlus
MutMap

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