MutS inhibits RecA-mediated strand exchange with platinated DNA substrates

Proceedings of the National Academy of Sciences of the United States of America
Melissa A Calmann, M G Marinus

Abstract

Human cell lines and Escherichia coli dam mutants are sensitive to the cytotoxic action of the anticancer agent, cisplatin. Introduction of mutations disabling DNA mismatch repair into these cell lines renders them resistant to the action of this drug. We used RecA-mediated strand exchange between homologous phiX174 molecules, one that was platinated and the other that was unmodified, to show that strand transfer is decreased in a dose-dependent manner. Transfer was severely decreased at 10 adducts per molecule (5,386 bp) and abolished with 24 adducts. At low levels of adduction, addition of MutS to the reaction further decreases the rate and yield in a dose-dependent manner. MutL addition was without effect even in the presence of MutS. The results suggest that although mismatch repair is beneficial for mutation avoidance, its antirecombination activity on inappropriate substrates can be lethal to the cell.

References

Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S S Su, P Modrich
Apr 29, 1982·Nature·P Karran, M G Marinus
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·L WorthP Modrich
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·D R DuckettP Modrich
Jan 1, 1996·Annual Review of Biochemistry·P Modrich, R Lahue
Aug 29, 1998·The Journal of Biological Chemistry·L WorthS Clark
Nov 14, 1998·Cell·M SeigneurB Michel
Feb 20, 1999·The Journal of Biological Chemistry·T H Wu, M G Marinus
Dec 30, 1999·Journal of Bacteriology·S J Sandler, K J Marians
Feb 9, 2000·Chemistry & Biology·Z Z ZdraveskiJ M Essigmann
Mar 15, 2000·Nature·M M CoxK J Marians
Apr 8, 2000·Trends in Biochemical Sciences·S C Kowalczykowski
Nov 28, 2000·Annual Review of Genetics·M D SuttonG C Walker
Dec 17, 2000·The Journal of Biological Chemistry·A Fabisiewicz, L Worth
Jun 19, 2001·Mutation Research·M Kartalou, J M Essigmann
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·F J López de Saro, M O'Donnell
Nov 14, 2001·The Journal of Biological Chemistry·Zoran Z ZdraveskiMartin G Marinus
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lawrence H Einhorn
Mar 1, 1957·Archives of Biochemistry and Biophysics·H ROSEN
Jun 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Neal SugawaraJames E Haber

❮ Previous
Next ❯

Citations

Jun 1, 2005·DNA Repair·Anetta Nowosielska, M G Marinus
Apr 2, 2005·Current Opinion in Microbiology·Anders Løbner-OlesenMartin G Marinus
Mar 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kevin Lehner, Sue Jinks-Robertson
Feb 26, 2005·Nucleic Acids Research·Melissa A CalmannM G Marinus
Jun 24, 2005·Nucleic Acids Research·Melissa A CalmannM G Marinus
Jan 8, 2016·DNA Repair·Khek-Chian ThamJoyce H G Lebbink
Sep 11, 2007·DNA Repair·Anetta Nowosielska, M G Marinus
Mar 3, 2010·Environmental and Molecular Mutagenesis·Karen M Vasquez
Feb 26, 2010·The New Phytologist·Alexandre Maréchal, Normand Brisson
Nov 17, 2015·Biochemistry. Biokhimii︠a︡·A S ErshovaA V Alexeevski
Jan 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xinjian LinStephen B Howell
Nov 1, 2012·EcoSal Plus·Bénédicte Michel, David Leach
Nov 1, 2012·EcoSal Plus·M G Marinus

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.