Mutual control of the PecS/PecM couple, two proteins regulating virulence-factor synthesis in Erwinia chrysanthemi

Molecular Microbiology
T PrailletWilliam Nasser

Abstract

The Erwinia chrysanthemi pecS mutant displays constitutive production of virulence factors, such as pectinases or cellulases. Complementation of the pecS mutation can be obtained in the presence of the pecS wild-type gene on a low-copy-number plasmid. Moreover, the resulting plasmid decreases the expression of a pecS::uidA chromosomal fusion, indicating the existence of an autoregulation mechanism. This negative autoregulation was confirmed and quantified by analysis of the pecS transcripts using primer-extension experiments. Band-shift assays and DNase I footprinting experiments demonstrated that the PecS protein could bind to the intergenic regulatory region, located between the pecS and pecM genes, with a relatively high affinity (apparent dissociation constant (K'[d]) close to 4nM). These PecS-binding sites overlap the pecS and pecM promoters. The comparison of these new PecS-binding sites with those previously characterized on the target genes confirms the absence of a consensus. This observation was in accordance with the results of the missing-contact experiments performed on the pecS-pecM intergenic regulatory region and the celZ operator. Concurrently, we demonstrated that the PecS protein negatively controls the expre...Continue Reading

Citations

May 27, 2009·Journal of Industrial Microbiology & Biotechnology·Sung-Soo ParkByung-Gee Kim
Aug 19, 2010·Journal of Molecular Cell Biology·Inoka C Perera, Anne Grove
Nov 7, 2008·Molecular Plant-microbe Interactions : MPMI·J D GlasnerA O Charkowski
Nov 9, 2002·Molecular Plant-microbe Interactions : MPMI·Thierry FranzaDominique Expert
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Anja Brencic, Stephen C Winans
Sep 7, 2012·Case Reports in Otolaryngology·Satoru KodamaMasashi Suzuki
Feb 2, 1999·Molecular Plant-microbe Interactions : MPMI·T FranzaD Expert
Aug 4, 2010·Journal of Molecular Biology·Inoka C Perera, Anne Grove
Mar 25, 2011·Protein Science : a Publication of the Protein Society·Inoka C Perera, Anne Grove
Jun 19, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Urszula L Burska, Jonathan N Fletcher
Feb 28, 2019·Scientific Reports·Nabanita BhattacharyyaAnne Grove

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.