Mutual regulation of cyclin-dependent kinase and the mitotic exit network.

The Journal of Cell Biology
Cornelia KönigE Schiebel

Abstract

The mitotic exit network (MEN) is a spindle pole body (SPB)-associated, GTPase-driven signaling cascade that controls mitotic exit. The inhibitory Bfa1-Bub2 GTPase-activating protein (GAP) only associates with the daughter SPB (dSPB), raising the question as to how the MEN is regulated on the mother SPB (mSPB). Here, we show mutual regulation of cyclin-dependent kinase 1 (Cdk1) and the MEN. In early anaphase Cdk1 becomes recruited to the mSPB depending on the activity of the MEN kinase Cdc15. Conversely, Cdk1 negatively regulates binding of Cdc15 to the mSPB. In addition, Cdk1 phosphorylates the Mob1 protein to inhibit the activity of Dbf2-Mob1 kinase that regulates Cdc14 phosphatase. Our data revise the understanding of the spatial regulation of the MEN. Although MEN activity in the daughter cells is controlled by Bfa1-Bub2, Cdk1 inhibits MEN activity at the mSPB. Consistent with this model, only triple mutants that lack BUB2 and the Cdk1 phosphorylation sites in Mob1 and Cdc15 show mitotic exit defects.

References

Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y Y LiK Bloom
May 1, 1993·The Journal of Cell Biology·P Y Goh, J V Kilmartin
Mar 14, 1998·Molecular Biology of the Cell·F C Luca, M Winey
Feb 28, 1998·The Journal of Cell Biology·R K Miller, M D Rose
Feb 9, 2000·Molecular Cell Biology Research Communications : MCBRC·R CenamorM Sánchez
Jun 6, 2000·Current Biology : CB·S L Jaspersen, D O Morgan
Feb 13, 2001·Trends in Cell Biology·D McCollum, K L Gould
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·A S MahR J Deshaies
Sep 21, 2001·Molecular and Cellular Biology·F C LucaM Winey
Oct 13, 2001·Molecular Biology of the Cell·R Visintin, A Amon
Apr 24, 2002·The Journal of Cell Biology·Gislene PereiraElmar Schiebel
Jun 6, 2002·The Journal of Biological Chemistry·Marco GeymonatSteven G Sedgwick
Aug 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jörg WiedenmannG Ulrich Nienhaus
Mar 15, 2003·The Journal of Biological Chemistry·Marco GeymonatSteven G Sedgwick
Oct 24, 2003·Nature·Jeffrey A UbersaxDavid O Morgan
Nov 8, 2003·Science·Gislene Pereira, Elmar Schiebel
Jan 14, 2004·Molecular Biology of the Cell·Jeffrey N MolkKerry Bloom
Dec 1, 2004·Annual Review of Genetics·Frank Stegmeier, Angelika Amon

❮ Previous
Next ❯

Citations

May 20, 2011·Molecular Biology of the Cell·Pilar Gutiérrez-EscribanoJaime Correa-Bordes
May 15, 2010·Cell Division·Jorrit M Enserink, Richard D Kolodner
Dec 15, 2010·Cell Division·Ayse K CaydasiGislene Pereira
Dec 18, 2013·PLoS Genetics·Barbara BaroEthel Queralt
Mar 7, 2014·Trends in Cell Biology·Manuel Hotz, Yves Barral
Jul 28, 2010·The Journal of Cell Biology·Romilde ManzoniRosella Visintin
Feb 16, 2011·The Journal of Cell Biology·Mauricio Valerio-Santiago, Fernando Monje-Casas
Jun 15, 2011·The Journal of Cell Biology·Daniela Trinca BertazziGislene Pereira
Jan 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nan-Peng ChenElmar Schiebel
Apr 19, 2012·Experimental Cell Research·Ayse Koca Caydasi, Gislene Pereira
May 15, 2012·Molecular Systems Biology·Ayse Koca CaydasiBashar Ibrahim
Apr 18, 2016·Cellular and Molecular Life Sciences : CMLS·Maria Angeles Juanes, Simonetta Piatti
Oct 29, 2011·Current Biology : CB·Marisa Segal
May 24, 2016·Cell Reports·Jose-Antonio Rodriguez-RodriguezEthel Queralt
Jun 24, 2016·Scientific Reports·Sun-Yong KimToshio Hakoshima
Jan 25, 2017·Nature Communications·Ayse Koca CaydasiGislene Pereira
Nov 1, 2016·Molecular Biology of the Cell·Jill E FalkAngelika Amon
Mar 17, 2010·The Journal of Biological Chemistry·Jiangtao MaTansy C Hammarton
Nov 6, 2014·The EMBO Journal·Thomas KuilmanFrank Uhlmann
Feb 20, 2020·Molecular Biology of the Cell·Ian W CampbellAngelika Amon
Jan 8, 2020·International Journal of Molecular Sciences·Yolanda Moyano-Rodriguez, Ethel Queralt
Apr 30, 2017·Cellular and Molecular Life Sciences : CMLS·Alina GoldsteinLarisa Gheber
Nov 13, 2020·PLoS Biology·Rowan S M HowellAttila Csikász-Nagy
Jan 23, 2021·ELife·Xiaoxue ZhouAngelika Amon
Aug 20, 2010·Journal of Cell Science·Annamaria Mocciaro, Elmar Schiebel
Mar 29, 2012·Journal of Cell Science·Saravanan PalaniGislene Pereira

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPase
nucleotide exchange
immunoprecipitation
fluorescence microscopy
immunoprecipitations

Software Mentioned

SoftWoRx
MetaMorph
ImageJ
Image Gauge
Adobe Photoshop

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.