Mutual structural effect of bilirubin and model membranes by vibrational circular dichroism

Biochimica Et Biophysica Acta
Pavlína NovotnáMarie Urbanová

Abstract

In this study, vibrational circular dichroism (VCD) spectroscopy was employed for the first time to study the bilirubin (BR) interaction with model membranes and models for membrane proteins. An enantioselective interaction of BR with zwitterionic 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and sphingomyelin (SPM) liposomes was observed by VCD and electronic circular dichroism (ECD) complemented by absorption and fluorescence spectroscopy. The M-form of BR was preferentially recognized in the BR/DMPC system at concentration above 1×10(-4)M, for lower concentrations the P-form of BR was recognized by the DMPC liposomes. The VCD spectra also showed that the SPM liposomes, which represent the main component of nerve cell membrane, were significantly more disturbed by the presence of BR than the DMPC liposomes-a stable association with a strong VCD signal was observed providing the explanations for the supposed BR neurotoxicity. The effect of time and pH on the BR/DMPC or SPM liposome systems was shown to be essential while the effect of temperature in the range of 15-70°C was negligible demonstrating the surprisingly high temperature stability of BR when interacting with the studied membranes. The influence of a membrane pr...Continue Reading

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Citations

Jun 15, 2015·Biophysical Chemistry·Pavlína NovotnáMarie Urbanová
Mar 10, 2015·Biochimica Et Biophysica Acta·Pavlína Novotná, Marie Urbanová
Jun 1, 2017·Physical Chemistry Chemical Physics : PCCP·Christian Merten
Sep 30, 2014·Magnetic Resonance in Chemistry : MRC·L F GaliullinaV V Klochkov
Sep 29, 2019·International Journal of Molecular Sciences·Ke ChenWei Wang
Sep 28, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Song-Di NiHong-Ming Ding

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