Mycobacteria infect different cell types in the human lung and cause species dependent cellular changes in infected cells

BMC Pulmonary Medicine
Dariimaa GanbatT Goldmann

Abstract

Mycobacterial infections remain a significant cause of morbidity and mortality worldwide. Due to limitations of the currently available model systems, there are still comparably large gaps in the knowledge about the pathogenesis of these chronic inflammatory diseases in particular with regard to the human host. Therefore, we aimed to characterize the initial phase of mycobacterial infections utilizing a human ex vivo lung tissue culture model designated STST (Short-Term Stimulation of Tissues). Human lung tissues from 65 donors with a size of 0.5-1 cm(3) were infected each with two strains of three different mycobacterial species (M. tuberculosis, M. avium, and M. abscessus), respectively. In order to preserve both morphology and nucleic acids, the HOPE® fixation technique was used. The infected tissues were analyzed using histo- and molecular-pathological methods. Immunohistochemistry was applied to identify the infected cell types. Morphologic comparisons between ex vivo incubated and non-incubated lung specimens revealed no noticeable differences. Viability of ex vivo stimulated tissues demonstrated by TUNEL-assay was acceptable. Serial sections verified sufficient diffusion of the infectious agents deep into the tissues. In...Continue Reading

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Citations

Dec 22, 2016·Cell and Tissue Research·Andreas C HockeStefan Hippenstiel
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Oct 7, 2021·Clinical Microbiology Reviews·Timothy D ShawCecilia M O'Kane

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Methods Mentioned

BETA
FCS
PCR

Software Mentioned

FixFoto
Image J
Infinity analyze
MATLAB
GraphPad
STST
SPSS
Graph Pad Prism

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