PMID: 9551681Apr 29, 1998Paper

Mycobacterial DNA not detected in liver sections from patients with primary biliary cirrhosis

Journal of Hepatology
J O'DonohueJ McFadden

Abstract

Recent studies in primary biliary cirrhosis have reported the detection of serum antibodies against Mycobacterium gordonae and of mycobacterial DNA in liver sections. The aim of this study was to investigate whether mycobacterial DNA is present in liver biopsy material in primary biliary cirrhosis. Archival liver biopsy specimens from 11 patients with primary biliary cirrhosis (10 female, mean age 52 years) and 11 patients with autoimmune hepatitis (10 female, mean age 53 years) were identified. Positive control tissue comprised five archival lymph node specimens from patients with tuberculous lymphadenopathy, three of which had stained positive on ZN staining, and also a liver biopsy specimen from a patient with tuberculous hepatitis (ZN positive). Fixed sections were deparaffinised and DNA was extracted by mechanical disruption with glass beads. DNA was purified by use of diatoms and lysis in guanidinium thiocyanate in a technique previously validated for archival DNA. Primers were directed to amplify a partial 16S ribosomal RNA gene yielding the species-specific character for mycobacteria, and also to amplify the constitutively-expressed human gene GAPDH. The polymerase chain reaction was shown to be capable of detecting 1 f...Continue Reading

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Citations

Apr 18, 2013·Immunologic Research·Dimitrios P BogdanosYehuda Shoenfeld
May 5, 2004·Journal of Hepatology·Ahmad S AbdulkarimKeith D Lindor
Nov 7, 1999·Journal of Hepatology·A TanakaM E Gershwin
Sep 25, 2012·World Journal of Gastroenterology : WJG·Daniel SmykDimitrios P Bogdanos
Jan 10, 2014·American Journal of Epidemiology·Richard J Q McNallyOliver F W James
Dec 26, 2012·Autoimmunity Reviews·Dimitrios P BogdanosYehuda Shoenfeld
Feb 14, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Fumi K VaryaniTimothy R Card
Aug 28, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Richard J Q McNallyOliver F W James
Dec 6, 2012·Tuberculosis Research and Treatment·Daniel S SmykEirini I Rigopoulou
Oct 7, 2006·Current Opinion in Gastroenterology·M Trauner, J L Boyer
May 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patrick S C LeungM Eric Gershwin
Oct 31, 2006·World Journal of Gastroenterology : WJG·Antonella MarangoniRoberto Cevenini

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