Mycobacterial Hsp65 antigen delivered by invasive Lactococcus lactis reduces intestinal inflammation and fibrosis in TNBS-induced chronic colitis model.

Scientific Reports
Vanessa Pecini da CunhaAnderson Miyoshi

Abstract

Intestinal fibrosis associated with Crohn's disease (CD), which a common and serious complication of inflammatory bowel diseases. In this context, heat shock proteins (HSPs) might serve as an alternative treatment because these antigens play important roles in the regulation of effector T cells. We thus evaluated the anti-inflammatory and antifibrotic capacities of an invasive and Hsp65-producing strain-Lactococcus lactis NCDO2118 FnBPA+ (pXYCYT:Hsp65)-in chronic intestinal inflammation to assess its potential as an alternative therapeutic strategy against fibrotic CD. Experimental colitis was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) in BALB/c mice, and the mice were treated orally with L. lactis NCDO2118 FnBPA+ (pXYCYT:Hsp65) via intragastric gavage. The oral administration of this strain significantly attenuated the severity of inflammation and intestinal fibrosis in mice (p < 0.05). These results are mainly justified by reductions in the levels of the pro-fibrotic cytokines IL-13 and TGF-β and increases in the concentration of the regulatory cytokine IL-10. The L. lactis NCDO2118 FnBPA+ (pXYCYT:Hsp65) strain contributed to reductions in the severity of inflammatory damage in chronic experimental CD, and these fi...Continue Reading

References

Oct 13, 2004·FEMS Microbiology Letters·Anderson MiyoshiVasco Azevedo
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexandra Zanin-ZhorovOfer Lider
Apr 2, 2005·Nature Reviews. Immunology·Willem van EdenBerent Prakken
Dec 23, 2008·Mucosal Immunology·S Fichtner-FeiglH-J Schlitt
Apr 7, 2009·Nature Reviews. Gastroenterology & Hepatology·Florian Rieder, Claudio Fiocchi
Dec 15, 2010·Trends in Immunology·Francisco J Quintana, Irun R Cohen
Apr 23, 2014·Nature Reviews. Immunology·Markus F Neurath
May 17, 2014·Inflammatory Bowel Diseases·Chao Li, John F Kuemmerle
Sep 2, 2014·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Puneet Kaur RandhawaAmteshwar Singh Jaggi
Mar 4, 2015·Nature Reviews. Gastroenterology & Hepatology·Ashwin N Ananthakrishnan
Nov 12, 2016·Central-European Journal of Immunology·Miroslav VasovicTatjana Kanjevac
Dec 5, 2016·Lancet·Joana TorresLaurent Peyrin-Biroulet
Feb 2, 2017·Nature Reviews. Gastroenterology & Hepatology·Markus F Neurath
Feb 15, 2017·Frontiers in Immunology·Ana Cristina Gomes-SantosAna Maria Caetano Faria
Jun 2, 2017·Nature Protocols·Stefan WirtzMarkus F Neurath
Jul 25, 2017·Frontiers in Medicine·Gerhard Rogler, Martin Hausmann
Aug 22, 2017·Frontiers in Medicine·Renata CurciarelloThomas T MacDonald
Sep 28, 2017·BMC Complementary and Alternative Medicine·Agustina Tri Endharti, Sofy Permana
Apr 29, 2018·Digestive Diseases and Sciences·Lei LianPing Lan
May 8, 2018·The Journal of Clinical Investigation·Alexandra Zanin-ZhorovIrun R Cohen
Aug 17, 2018·Nature Communications·Georg Schett, Markus F Neurath
Mar 8, 2019·Autoimmunity Reviews·Paolo GiuffridaAntonio Di Sabatino
Sep 26, 2019·Seminars in Immunopathology·Heike SchmittRaja Atreya
Nov 20, 2019·Mucosal Immunology·Oliver Pabst, Emma Slack
Jan 17, 2020·Inflammatory Bowel Diseases·Raditya IswandanaPeter Olinga
Feb 7, 2020·Nutrients·Paola BalestrieriMichele Cicala
Jun 21, 2020·Nature Reviews. Disease Primers·Giulia RodaSilvio Danese

❮ Previous
Next ❯

Citations

May 21, 2021·Journal of Digestive Diseases·Xiao Xuan LinRen Mao

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resections
ELISA
lavage

Software Mentioned

GraphPad Prism
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.