Mycobacteriophage CRB2 defines a new subcluster in mycobacteriophage classification

PloS One
Cristian Alejandro SuarezHéctor R Morbidoni

Abstract

Mycobacteriophages are viruses -mostly temperates- that infect Mycobacterium smegmatis and sometimes Mycobacterium tuberculosis. Mycobacteriophages are grouped in clusters on the basis of the overall nucleotide sequence homology, being further divided in subclusters as more mycobacteriophage genomes are sequenced and annotated. As part of our on-going screening for novel isolates, we herein report the bioinformatics analysis of CRB2, a mycobacteriophage belonging into the Siphoviridae family that propagates at 30°C. CRB2 has a 72,217 bp genome with a 69.78% GC content that belongs to Cluster B; nucleotide comparison with other B cluster members positions CRB2 as the sole member of a new subcluster, B9, being mycobacteriophage Saguaro (belonging into subcluster B7) its closest relative. Sequencing and annotation of 14 mycobacteriophages isolated by our group has yielded six cluster A members, a singleton, four of the five members of subcluster B6, one of the three reported members of subcluster G4, and CRB2 which defines subcluster B9. Considering the massive mycobacteriophage search performed in USA and the relatively rarity of our phages, we propose that factors other than size of the sampling determine the variability of myco...Continue Reading

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Citations

Jan 17, 2021·International Journal of Molecular Sciences·Anna Allué-GuardiaJordi B Torrelles

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Datasets Mentioned

BETA
MK059749

Methods Mentioned

BETA
PCR
electrophoresis
PCA

Software Mentioned

SplitsTree
GeneMark
DNAMaster
Gegenees
MEGA
HHPred
Phamerator
gplots
tRNA Scan SE
Glimmer

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