Mycobacteriophage Fruitloop gp52 inactivates Wag31 (DivIVA) to prevent heterotypic superinfection

Molecular Microbiology
Ching-Chung Ko, G F Hatfull

Abstract

Bacteriophages engage in complex dynamic interactions with their bacterial hosts and with each other. Bacteria have numerous mechanisms to resist phage infection, and phages must co-evolve by overcoming bacterial resistance or by choosing an alternative host. Phages also compete with each other, both during lysogeny by prophage-mediated defense against viral attack and by superinfection exclusion during lytic replication. Phages are enormously diverse genetically and are replete with small genes of unknown function, many of which are not required for lytic growth, but which may modulate these bacteria-phage and phage-phage dynamics. Using cellular toxicity of phage gene overexpression as an assay, we identified the 93-residue protein gp52 encoded by Cluster F mycobacteriophage Fruitloop. The toxicity of Fruitloop gp52 overexpression results from interaction with and inactivation of Wag31 (DivIVA), an essential Mycobacterium smegmatis protein organizing cell wall biosynthesis at the growing cellular poles. Fruitloop gene 52 is expressed early in lytic growth and is not required for normal Fruitloop lytic replication but interferes with Subcluster B2 phages such as Hedgerow and Rosebush. We conclude that Hedgerow and Rosebush are...Continue Reading

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Citations

Feb 28, 2019·PloS One·Cristian Alejandro SuarezHéctor Ricardo Morbidoni
Aug 14, 2019·Journal of Bacteriology·Lauren R HammondPrahathees J Eswara
Sep 30, 2020·Annual Review of Virology·Graham F Hatfull
Dec 4, 2019·Critical Reviews in Biotechnology·Fatemeh SafariYounes Ghasemi
Oct 31, 2019·Transcription·Markus C Wahl, Ranjan Sen
Jan 17, 2020·Nature·Hannah G HamptonPeter C Fineran
Jan 17, 2021·International Journal of Molecular Sciences·Anna Allué-GuardiaJordi B Torrelles
Oct 29, 2020·Current Opinion in Biotechnology·Lucia M MalonePeter C Fineran
Mar 26, 2021·Scientific Reports·Katherine S WetzelGraham F Hatfull
Apr 12, 2021·Biochemistry. Biokhimii︠a︡·Artem B IsaevKonstantin V Severinov
Oct 7, 2018·Microbiology Spectrum·Graham F Hatfull

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