Mycobacteriophage Marvin: a new singleton phage with an unusual genome organization.

Journal of Virology
Catherine MageeneyG F Hatfull

Abstract

Mycobacteriophages represent a genetically diverse group of viruses that infect mycobacterial hosts. Although more than 80 genomes have been sequenced, these still poorly represent the likely diversity of the broader population of phages that can infect the host, Mycobacterium smegmatis mc(2)155. We describe here a newly discovered phage, Marvin, which is a singleton phage, having no previously identified close relatives. The 65,100-bp genome contains 107 predicted protein-coding genes arranged in a noncanonical genomic architecture in which a subset of the minor tail protein genes are displaced about 20 kbp from their typical location, situated among nonstructural genes anticipated to be expressed early in lytic growth. Marvin is not temperate, and stable lysogens cannot be recovered from infections, although the presence of a putative xis gene suggests that Marvin could be a relatively recent derivative of a temperate parent. The Marvin genome is replete with novel genes not present in other mycobacteriophage genomes, and although most are of unknown function, the presence of amidoligase and glutamine amidotransferase genes suggests intriguing possibilities for the interactions of Marvin with its mycobacterial hosts.

References

Sep 1, 1974·Virology·R W Hendrix, S R Casjens
Dec 1, 1984·Cell·I Katsura, R W Hendrix
Jan 1, 1993·Bio Systems·M Borodovsky, J McIninch
Sep 1, 1995·Molecular Microbiology·C E NesbitG F Hatfull
Sep 1, 1997·Nucleic Acids Research·M Belfort, R J Roberts
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·V Derbyshire, M Belfort
Jun 24, 1998·Journal of Molecular Biology·M E FordG F Hatfull
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·R W HendrixG F Hatfull
Nov 11, 1999·Nucleic Acids Research·A L DelcherS L Salzberg
Jan 25, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·M E FordG F Hatfull
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·K E Wommack, R R Colwell
Dec 21, 2000·Trends in Microbiology·R W HendrixS Casjens
Dec 21, 2000·Molecular Microbiology·J MediavillaG F Hatfull
Jan 17, 2002·Cell·Harald Brüssow, Roger W Hendrix
Oct 9, 2002·Genome Research·Lincoln D SteinSuzanna Lewis
Apr 23, 2003·Cell·Marisa L PedullaGraham F Hatfull
Jun 12, 2003·Research in Microbiology·Roger W HendrixMargaret C M Smith
Oct 24, 2003·Current Opinion in Microbiology·Roger W Hendrix
Jan 6, 2004·Nucleic Acids Research·Dean Laslett, Bjorn Canback
Jul 16, 2005·Current Opinion in Microbiology·Sherwood R Casjens
Jun 23, 2006·PLoS Genetics·Graham F HatfullRoger W Hendrix
Feb 21, 2007·Bioinformatics·Jan KrumsiekThomas Rattei
Sep 15, 2007·Nature Reviews. Microbiology·Curtis A Suttle
Oct 13, 2007·Virology·Jenny R ChangTerje Dokland
Feb 26, 2008·Journal of Molecular Biology·Dhruti SavaliaKonstantin Severinov
Jan 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Peter C FineranGeorge P C Salmond
May 15, 2009·Bioinformatics·Ed LeeSuzanna Lewis
Jun 27, 2009·Molecular Microbiology·Kimberly PayneGraham F Hatfull
Jun 10, 2010·Annual Review of Microbiology·Graham F Hatfull
Dec 25, 2010·Applied and Environmental Microbiology·Steve PetrovskiDaniel Tillett

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Citations

Nov 29, 2013·Genome Announcements·Graham F HatfullUNKNOWN Phage Hunters Integrating Research and Education (PHIRE) Program
Jul 23, 2013·PloS One·Welkin H PopeGraham F Hatfull
Nov 3, 2014·Annual Review of Virology·Graham F Hatfull, Vincent Racaniello
Jul 9, 2016·Genome Announcements·Welkin H PopeGraham F Hatfull
Dec 12, 2013·Journal of Labelled Compounds & Radiopharmaceuticals·Jason P HollandNeil Vasdev
Sep 30, 2020·Annual Review of Virology·Graham F Hatfull
Jun 16, 2018·Journal of Microbiology & Biology Education·Michael J WolyniakDaniel E Westholm
Feb 1, 2014·Microbiology Spectrum·Madalena Pimentel
Oct 7, 2018·Microbiology Spectrum·Graham F Hatfull

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