Mycobacterium tuberculosis 6C sRNA binds multiple mRNA targets via C-rich loops independent of RNA chaperones

Nucleic Acids Research
Juntao MaiJun Liu

Abstract

Bacterial small regulatory RNAs (sRNAs) are the most abundant class of post-transcriptional regulators and have been well studied in Gram-negative bacteria. Little is known about the functions and mechanisms of sRNAs in high GC Gram-positive bacteria including Mycobacterium and Streptomyces. Here, we performed an in-depth study of 6C sRNA of Mycobacterium tuberculosis, which is conserved among high GC Gram-positive bacteria. Forty-seven genes were identified as possible direct targets of 6C sRNA and 15 of them were validated using an in vivo translational lacZ fusion system. We found that 6C sRNA plays a pleotropic role and regulates genes involved in various cellular processes, including DNA replication and protein secretion. Mapping the interactions of 6C sRNA with mRNA targets panD and dnaB revealed that the C-rich loops of 6C sRNA act as direct binding sites to mRNA targets. Unlike in Gram-negative bacteria where RNA binding proteins Hfq and ProQ are required, the interactions of 6C sRNA with mRNAs appear to be independent of RNA chaperones. Our findings suggest that the multiple G-C pairings between single stranded regions are sufficient to establish stable interactions between 6C sRNA and mRNA targets, providing a mechani...Continue Reading

References

Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·C M SassettiE J Rubin
Aug 31, 2002·Nucleic Acids Research·Xueguang SunRoger M Wartell
Dec 14, 2002·Microbiology·Rebecca GreendykeMurty V V S Madiraju
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Oct 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sarah A StanleyJeffery S Cox
Oct 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tsungda HsuWilliam R Jacobs
Mar 11, 2004·Molecular Microbiology·Poul Valentin-HansenChristina Udesen
Mar 31, 2007·Current Opinion in Microbiology·Richard G Brennan, Todd M Link
Oct 9, 2007·Nature Reviews. Microbiology·Abdallah M AbdallahWilbert Bitter
May 15, 2008·BMC Genomics·Josef PánekJirí Vohradský
Aug 8, 2008·Nature·Sridharan RaghavanJeffery S Cox
Sep 2, 2008·Journal of Bacteriology·Jesús Gonzalo-AsensioCarlos Martín
Nov 15, 2008·Nucleic Acids Research·Julia P SwierczMarie A Elliot
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Jun 27, 2009·Molecular Microbiology·Kristine B Arnvig, Douglas B Young
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Blair R G GordonJun Liu
Mar 4, 2010·Genome Biology·Mark D Robinson, Alicia Oshlack
Apr 28, 2011·RNA Biology·Michael-Paul VockenhuberBeatrix Suess
Jul 16, 2011·Nature Reviews. Microbiology·Jörg Vogel, Ben F Luisi
Sep 20, 2011·Molecular Cell·Gisela StorzKaren M Wassarman
Nov 26, 2011·Algorithms for Molecular Biology : AMB·Ronny LorenzIvo L Hofacker
Mar 23, 2012·Critical Reviews in Microbiology·Patricio Sobrero, Claudio Valverde
May 2, 2012·RNA Biology·Kristine Arnvig, Douglas Young
Nov 13, 2012·Trends in Genetics : TIG·Guillaume DesnoyersEric Massé
Jan 1, 2013·Tuberculosis·Michael A DeJesusThomas R Ioerger
Jan 8, 2013·Tuberculosis·Gyanu LamichhaneKathleen A McDonough
Mar 1, 2014·RNA Biology·Sabine Brantl, Reinhold Brückner
Mar 29, 2014·RNA Biology·Nona HeueisBeatrix Suess
Aug 12, 2014·Frontiers in Cellular and Infection Microbiology·Katie HaningLydia M Contreras
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber

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Citations

Jul 3, 2020·Frontiers in Molecular Biosciences·Juan José González Plaza
Apr 9, 2021·Biochemistry. Biokhimii︠a︡·Albina A OstrikElena G Salina
Sep 23, 2021·Microbiology Spectrum·Joanna HoughtonKristine B Arnvig
Nov 23, 2021·Frontiers in Microbiology·Yingyu ChenAizhen Guo
Dec 30, 2021·RNA Biology·Kratika Naskulwar, Lourdes Peña-Castillo
Jan 5, 2022·Current Microbiology·Karen L F Alvarez-ErasoAndres Baena

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Datasets Mentioned

BETA
GSE119364

Methods Mentioned

BETA
co-immunoprecipitation
RNA-seq
PCR
electrophoresis
electrophoretic mobility shift assay
in vitro transcription
saturation binding

Software Mentioned

edgeR
Mfold
HTSeq
RNAfold
ImageJ
Prism
Seqtk
Bowtie2
IntaRNA

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