Abstract
Mycobacterium bovis frequently infects wild and farm deer species with tuberculosis. This study investigated mycobacterial infection in two native deer species Cervus unicolor swinhoei (Formosan Sambar, Sambar) and C. nippon taiouanus (Formasan Sika, Sika). Based on different sampling sources of 19 intradermal tuberculin test (ITT) Sambar, mycobacterial infection and/or species were detected by acid-fast stain, duplex polymerase chain reaction (PCR) and multiplex nested PCR (mnPCR) methods, traditional mycobacterial culture and gross lesion. Blood samples of 167 Sambar deer and 147 Sika deer were then tested by duplex PCR and mnPCR methods to investigate the prevalence of mycobacterial infection. Sequence variations of these mycobacterial species were analyzed as well. Duplex PCR and mnPCR assays could differentiate between MTBC (M. bovis and M. tuberculosis) and M. avium, as well as between M. bovis and M. tuberculosis, respectively. These PCR methods showed a higher detection rate than traditional culture and matched the gross lesions examined in 19 ITT-examined Sambar. Therefore, the mycobacterial infection in blood samples of 314 deer samples was detected using these PCR methods. Duplex PCR and mnPCR showed an identical pre...Continue Reading
References
Nov 4, 1978·The Veterinary Record·J FrancisA J Frost
Sep 1, 1992·Infection·M LibanoreF Ghinelli
May 1, 1990·The Journal of Infectious Diseases·K D EisenachJ T Crawford
Jan 29, 1988·Science·R K SaikiH A Erlich
Sep 1, 1995·Microbiology·J G RodriguezL A Murillo
Aug 1, 1995·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·L M O'Reilly, C J Daborn
Jan 7, 1998·The Veterinary Record·F C QuigleyJ Egan
May 29, 1999·Science·M A BehrP M Small
Jun 29, 2002·Journal of Clinical Microbiology·Linda M ParsonsMax Salfinger
Oct 2, 2002·Molecular Microbiology·Yu-Tze HorngPaul Williams
Mar 9, 2004·Journal of Clinical Microbiology·Bum-Joon KimYoon-Hoh Kook
Nov 16, 2004·Veterinary Immunology and Immunopathology·M ThomC J Howard
Aug 1, 1985·New Zealand Veterinary Journal·G W de Lisle, P F Havill
Dec 1, 1987·New Zealand Veterinary Journal·K C CorrinG W de Lisle
Nov 8, 2005·Journal of Clinical Microbiology·Shunji TakakuraSatoshi Ichiyama
Dec 13, 2005·Tuberculosis·J F T GriffinC G Mackintosh
Mar 7, 2006·Journal of Microbiological Methods·Po-Chi SooHsin-Chih Lai
Citations
Mar 26, 2014·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Fang-Lan YuMing-Chih Yu
Jun 3, 2021·Pathogens·Rebecca Thomas, Mark Chambers