Mycobacterium tuberculosis employs Cpn60.2 as an adhesin that binds CD43 on the macrophage surface

Cellular Microbiology
Tyler B M HickeyRichard W Stokes

Abstract

CD43 is a large sialylated glycoprotein found on the surface of haematopoietic cells and has been previously shown to be necessary for efficient macrophage binding and immunological responsiveness to Mycobacterium tuberculosis. Using capsular material from M. tuberculosis and recombinant CD43-Fc, we have employed affinity chromatography to show that Cpn60.2 (Hsp65, GroEL), and to a lesser extent DnaK (Hsp70), bind to CD43. Competitive inhibition using recombinant protein and polyclonal F(ab')(2) antibody-mediated epitope masking studies were used to evaluate M. tuberculosis binding to CD43(+/+) versus CD43(-/-) macrophages. Results showed that Cpn60.2, but not DnaK, acts as a CD43-dependent mycobacterial adhesin for macrophage binding. Assessment of the specific binding between Cpn60.2 and CD43 showed it to be saturable, with a comparatively weak affinity in the low micromolar range. We have also shown that the ability of Cpn60.2 to competitively inhibit M. tuberculosis binding to macrophages is shared by the Escherichia coli homologue, GroEL, but not by the mouse and human Hsp60 homologues. These findings add to a growing field of research that implicates molecular chaperones as having extracellular functions, including bacter...Continue Reading

References

Apr 15, 1989·Cellular Immunology·R KellerR Keist
Jan 1, 1993·Immunogenetics·C M Baecher-AllanJ G Frelinger
Dec 1, 1996·American Journal of Respiratory Cell and Molecular Biology·S ZimmerliJ D Ernst
Apr 7, 1998·Infection and Immunity·J D Ernst
Aug 11, 1998·Clinical and Experimental Immunology·B TaşçiG Saruhan-Direskeneli
Dec 29, 1998·Immunology Today·J R OstbergJ G Frelinger
Jan 9, 1999·Clinical Microbiology Reviews·U Zügel, S H Kaufmann
Nov 26, 1999·Biochimica Et Biophysica Acta·M Fukuda, S Tsuboi
Jul 19, 2000·The Journal of Experimental Medicine·C FratazziH G Remold
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D A CarlowH J Ziltener
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T K van den BergP R Crocker
Jan 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christiane HabichVolker Burkart
Jul 31, 2002·Molecular Pathology : MP·J C Ranford, B Henderson
Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kristin H LongSimon L Newman
Sep 1, 1952·The British Journal of Dermatology·D SHARVILL
Mar 1, 1952·American Journal of Clinical Pathology·G GOMORI
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Valerie J QuesniauxBernhard Ryffel
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·April K RandhawaRichard W Stokes
Oct 26, 2005·Biochemical and Biophysical Research Communications·Dina Montufar-SolisJohn R Klein
Oct 29, 2005·Tuberculosis·Rohini QamraBrian Henderson
Jun 23, 2006·Infection and Immunity·Brian HendersonAnthony R M Coates
Oct 20, 2006·Vaccine·Khaleda Rahman QaziCarmen Fernández
Sep 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Antonio Gigliotti RothfuchsAlan Sher
Oct 20, 2007·Nature Reviews. Immunology·Antonella Viola, Neetu Gupta
Mar 27, 2010·Tuberculosis·Brian HendersonAnthony R M Coates

❮ Previous
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Citations

Jan 11, 2012·Clinical Microbiology Reviews·Alexander Harms, Christoph Dehio
May 17, 2014·PLoS Pathogens·Jacqueline L Naffin-OlivosJyothi Rengarajan
May 17, 2014·Biology·Veera Kainulainen, Timo K Korhonen
Apr 5, 2013·Biological Reviews of the Cambridge Philosophical Society·Brian HendersonPeter A Lund
Nov 18, 2014·Biochemical Society Transactions·Brian Henderson
May 20, 2015·Cell Stress & Chaperones·C M Santosh KumarGaurang Mahajan
Nov 26, 2013·Biochimica Et Biophysica Acta·Marie-Pierre Castanié-CornetPierre Genevaux
Aug 1, 2014·American Journal of Respiratory Cell and Molecular Biology·Monica CampoThomas R Hawn
Jan 11, 2013·Memórias do Instituto Oswaldo Cruz·André Alves DiasMaria Cristina Vidal Pessolani
Nov 15, 2017·Infection and Immunity·Maria GeorgievaJyothi Rengarajan
Aug 4, 2018·Journal of Medical Microbiology·Ciamak Ghazaei, Marie Line El Helou
Jun 15, 2019·Scientific Reports·Frederico Alisson-SilvaAdriane R Todeschini
Jun 12, 2020·The Journal of Microbiology·Kesavan DineshkumarHuaxi Xu
Mar 23, 2017·Journal of Immunology Research·Nadia Martinez-Martin
Nov 16, 2017·Protein Science : a Publication of the Protein Society·Flavia SquegliaRita Berisio
Nov 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tawanda ZiningaAddmore Shonhai
Oct 2, 2019·The Indian Journal of Medical Research·Durga Bisht, Laxman S Meena
Jul 20, 2019·The Biochemical Journal·Rainer KalscheuerRafael Prados-Rosales
Oct 2, 2019·Frontiers in Molecular Biosciences·Alyssa M McQuillanRichard D Cummings
Jan 11, 2020·Frontiers in Chemistry·Chao GaoRichard D Cummings
Dec 17, 2019·Microbiological Research·Vivek VinodRaja Biswas
Jun 9, 2019·Biochimica Et Biophysica Acta. General Subjects·Chih-Jung KuoChun-Chi Chen
Jun 11, 2021·PloS One·Jessica RauchAnke Osterloh

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