Mycobacterium tuberculosis H37Rv expresses differential proteome during intracellular survival within alveolar epithelial cells compared with macrophages

Pathogens and Disease
S AgarwalIndu Verma

Abstract

Mycobacterium tuberculosis survives within the infected host cells by escaping phagolysosomal fusion and adaptation to hosts' intracellular milieu. Recently, the role of alveolar epithelial cells (AECs) in the survival of intracellular M. tuberculosis has gained importance. It has been reported that M. tuberculosis causes cytotoxicity in AECs, a phenotype attributed to M. tuberculosis virulence that could be due to the differential gene/protein expression of bacilli in these cells. Thus, the present study focused on comparative proteomic analysis of intracellular mycobacteria within macrophages and epithelial cells. Intracellular mycobacteria from infected alveolar epithelial (A549) and macrophage (THP-1) cell lines were harvested, and two-dimensional polyacrylamide gel electrophoresis of mycobacterial proteins was performed. The protein spots of interest were picked and MALDI-TOF/MS was performed to identify proteins. Differential expression of 11 mycobacterial proteins was found in infected AECs, out of which six proteins were confirmed by MS/MS analysis. The identified proteins mainly belonged to functional categories of virulence, detoxification and adaptation, and are known to take part in metabolism and respiration, maint...Continue Reading

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A ShevchenkoM Mann
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·D R ShermanG K Schoolnik
Mar 22, 2002·Cellular Microbiology·Wandy L BeattyDavid G Russell
Jan 1, 2003·The Journal of Infectious Diseases·Kaeryn N LewisDavid R Sherman
Mar 5, 2003·Pharmacological Reviews·Michael R Yeaman, Nannette Y Yount
Jul 7, 2005·Genome Biology·Ping Wang, Joseph Heitman
Mar 21, 2006·Proteomics·Jens MattowUlrich E Schaible
Dec 6, 2008·PLoS Pathogens·Nguyen Thuy Thuong ThuongThomas R Hawn
Feb 17, 2009·Journal of Proteome Research·Julie LamontagneEustache Paramithiotis
Oct 24, 2009·FEBS Letters·Tobias MaierLuis Serrano
Nov 13, 2009·Molecular Microbiology·Arvind G KinhikarSuman Laal
Nov 19, 2010·PloS One·Nicole A KruhKaren M Dobos
Jun 21, 2011·Journal of Biophysics·Fatemeh MadaniAstrid Gräslund
Mar 10, 2012·Database : the Journal of Biological Databases and Curation·Ankur GautamG P S Raghava
Mar 23, 2013·Journal of Translational Medicine·Ankur GautamGajendra P S Raghava
Jul 24, 2015·Methods in Molecular Biology·Ankur GautamGajendra Pal Singh Raghava
Apr 23, 2016·Cell·Yansheng LiuRuedi Aebersold

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