Mycobacterium tuberculosis invades and replicates within type II alveolar cells.

Infection and Immunity
L E Bermudez, Joseph Goodman

Abstract

Although Mycobacterium tuberculosis is assumed to infect primarily alveolar macrophages after being aspirated into the lung in aerosol form, it is plausible to hypothesize that M. tuberculosis can come in contact with alveolar epithelial cells upon arrival into the alveolar space. Therefore, as a first step toward investigation of the interaction between M. tuberculosis and alveolar epithelial cells, we examined the ability of M. tuberculosis to bind to and invade alveolar epithelial cells in vitro. The H37Rv and H37Ra strains of M. tuberculosis were cultured to mid-log phase and used in both adherence and invasion assays. The A549 human type II alveolar cell line was cultured to confluence in RPMI 1640 supplemented with 5% fetal bovine serum, L-glutamine, and nonessential amino acids. H37Rv was more efficient in entering A549 cells than H37Ra, Mycobacterium avium, and Escherichia coli Hb101, and nonpiliated strain (4.7% +/- 1.0% of the initial inoculum in 2 h compared with 3.1% +/- 0.8%, 2.1% +/- 0.9%, and 0.03% +/- 0.0%, respectively). The invasion was more efficient at 37 degrees C than 30 degrees C (4.7% +/- 1.0% compared with 2.3% +/- 0.8%). H37Rv and H37Ra were both capable of multiplying intracellularly at a similar rati...Continue Reading

References

Aug 21, 1992·Science·B R Bloom, C J Murray
Mar 1, 1989·Reviews of Infectious Diseases·D W Smith, E H Wiegeshaus
Dec 1, 1989·Infection and Immunity·E WiegeshausD W Smith
Nov 1, 1989·The Journal of Experimental Medicine·E L Racoosin, J A Swanson
Jan 1, 1983·The Journal of Cell Biology·R M SteinmanZ A Cohn
Jul 1, 1984·Infection and Immunity·M E Mapother, J G Songer
Mar 1, 1995·Infection and Immunity·S G FillerP M Luckett
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T A OelschlaegerD J Kopecko
May 1, 1993·Infection and Immunity·T L RatliffE J Brown
May 1, 1993·American Journal of Respiratory Cell and Molecular Biology·S A MetteS M Albelda
Nov 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·C C SHEPARD

❮ Previous
Next ❯

Citations

Jun 25, 1998·American Journal of Respiratory and Critical Care Medicine·K MarutaS Yoshida
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·F D MenozziC Locht
Sep 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kathleen M Wright, Jon S Friedland
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kevin PetheFranco D Menozzi
Mar 26, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Stephen P PerfettoMario Roederer
Oct 27, 2007·Expert Opinion on Biological Therapy·Camille LochtFrançoise Mascart
Jan 15, 2011·Clinical & Developmental Immunology·Suhail Ahmad
Mar 27, 2012·The Journal of Infectious Diseases·Rebecca Axelsson-RobertsonMarkus Maeurer
Mar 25, 2015·Immunology and Cell Biology·Philippa J RandallMuazzam Jacobs
Nov 4, 2016·EMBO Molecular Medicine·Natalie ZimmermannHedda Wardemann
Feb 23, 2017·Frontiers in Cellular and Infection Microbiology·Qing ZhengXiaoke Hao
Jun 20, 2020·Journal of Leukocyte Biology·Tamara Silva RodriguesVânia Luiza Deperon Bonato
Feb 7, 2002·Expert Opinion on Investigational Drugs·André Bryskier, John Lowther
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Samira MuñozJose Antonio Enciso
Sep 1, 2005·Protein Science : a Publication of the Protein Society·Javier GarcíaManuel E Patarroyo
Dec 11, 2007·Cellular Microbiology·Brooke H RussellYi Xu
Jan 30, 2004·International Journal of Experimental Pathology·Lee-Anne StantonSiamon Gordon
Oct 16, 2008·In Vitro Cellular & Developmental Biology. Animal·I H Lelong-RebelG Rebel
Nov 16, 2010·American Journal of Respiratory and Critical Care Medicine·Stephan Schwander, Keertan Dheda
Mar 28, 2008·Scandinavian Journal of Immunology·B Rivas-SantiagoR Hernández-Pando
Apr 11, 2014·Journal of Internal Medicine·A ZumlaM Maeurer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.