Mycobacterium tuberculosis Rv1302 and Mycobacterium smegmatis MSMEG_4947 have WecA function and MSMEG_4947 is required for the growth of M. smegmatis

FEMS Microbiology Letters
Yue JinYufang Ma

Abstract

The disaccharide d-N-acetylglucosamine-l-rhamnose plays an important role in the mycobacterial cell wall as a linker connecting arabinogalactan and peptidoglycan via a phosphodiester linkage. The first step of the disaccharide linker is the formation of decaprenyl phosphate-GlcNAc, which is catalyzed by GlcNAc-1-phosphate transferase. In Gram-negative bacteria, the wecA gene specifies the UDP-GlcNAc: undecaprenyl phosphate GlcNAc-1-phosphate transferase (WecA), which catalyzes the first step in the biosynthesis of lipopolysaccharide O-antigen. Mycobacterium tuberculosis Rv1302 and Mycobacterium smegmatis MSMEG_4947 show homology to Escherichia coli WecA protein. We cloned Rv1302 and MSMEG_4947 and introduced plasmids pYJ-1 (carrying Rv1302) and pYJ-2 (carrying MSMEG_4947) into a wecA-defective strain of E. coli MV501, respectively. Lipopolysaccharide analysis demonstrated that lipopolysaccharide synthesis in MV501 (pYJ-1) and MV501 (pYJ-2) was restored upon complementation with Rv1302 and MSMEG_4947, respectively. This provides the first evidence that Rv1302 and MSMEG_4947 have the same function as E. coli WecA. We also generated an M. smegmatis MSMEG_4947 knockout mutant using a homologous recombination strategy. The disruptio...Continue Reading

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Citations

Jun 11, 2014·Critical Reviews in Biochemistry and Molecular Biology·Shiva Kumar AngalaMary Jackson
Oct 22, 2015·Annual Review of Microbiology·Monika JankuteGurdyal S Besra
Dec 16, 2016·Parasitology·Katherine A Abrahams, Gurdyal S Besra
Sep 7, 2017·Antimicrobial Agents and Chemotherapy·Stanislav HuszárKatarína Mikušová
Sep 1, 2019·The Journal of Antibiotics·Yoshimasa IshizakiMasayuki Igarashi
Oct 1, 2020·The Cell Surface·Sarah M BattGurdyal S Besra
Jun 25, 2015·Microbiology Spectrum·Monika JankuteGurdyal S Besra
Nov 4, 2010·ACS Chemical Biology·Wenlan ChenPeng George Wang

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