Mycobacterium tuberculosis-specific CD4+ and CD8+ T cells differ in their capacity to recognize infected macrophages

PLoS Pathogens
Jason D YangSamuel M Behar

Abstract

Containment of Mycobacterium tuberculosis (Mtb) infection requires T cell recognition of infected macrophages. Mtb has evolved to tolerate, evade, and subvert host immunity. Despite a vigorous and sustained CD8+ T cell response during Mtb infection, CD8+ T cells make limited contribution to protection. Here, we ask whether the ability of Mtb-specific T cells to restrict Mtb growth is related to their capacity to recognize Mtb-infected macrophages. We derived CD8+ T cell lines that recognized the Mtb immunodominant epitope TB10.44-11 and compared them to CD4+ T cell lines that recognized Ag85b240-254 or ESAT63-17. While the CD4+ T cells recognized Mtb-infected macrophages and inhibited Mtb growth in vitro, the TB10.4-specific CD8+ T cells neither recognized Mtb-infected macrophages nor restricted Mtb growth. TB10.4-specific CD8+ T cells recognized macrophages infected with Listeria monocytogenes expressing TB10.4. However, over-expression of TB10.4 in Mtb did not confer recognition by TB10.4-specific CD8+ T cells. CD8+ T cells recognized macrophages pulsed with irradiated Mtb, indicating that macrophages can efficiently cross-present the TB10.4 protein and raising the possibility that viable bacilli might suppress cross-presenta...Continue Reading

References

Jan 20, 2000·Infection and Immunity·L BrandtP Andersen
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·N V SerbinaJ L Flynn
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D M LewinsohnM R Alderson
Feb 13, 2001·Infection and Immunity·K A BodnarJ L Flynn
Feb 13, 2001·Infection and Immunity·M Gonzalez-Juarrero, I M Orme
Jul 23, 2003·Nature Medicine·Ulrich E SchaibleStefan H E Kaufmann
Aug 6, 2003·Current Opinion in Immunology·JoAnne L Flynn, John Chan
Sep 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mercedes Gonzalez-JuarreroIan M Orme
Sep 13, 2003·American Journal of Respiratory and Critical Care Medicine·Deborah A LewinsohnDavid M Lewinsohn
Nov 30, 2004·The Lancet Infectious Diseases·Madhukar PaiJohn M Colford
Jan 18, 2006·Immunity·Florian WinauUlrich E Schaible
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arati KamathSamuel M Behar
Apr 5, 2007·Nature Protocols·Jeff HolstDario A A Vignali
Sep 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rolf BilleskovJes Dietrich
Jul 2, 2008·Journal of Leukocyte Biology·Rajeev Mani NepalPaula Bryant
Sep 10, 2008·The Journal of Experimental Medicine·Alena M GallegosMichael S Glickman
Oct 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Markus Sköld, Samuel M Behar
Jun 30, 2009·Nature Immunology·Maziar DivangahiHeinz G Remold
May 25, 2010·Nature Genetics·Iñaki ComasSebastien Gagneux
Jun 16, 2010·The Journal of Experimental Medicine·Shahin ShafianiKevin B Urdahl
Jul 14, 2010·Nature Immunology·Maziar DivangahiSamuel M Behar
Aug 31, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeff E GrotzkeDavid M Lewinsohn
Dec 24, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joshua S WoodworthSamuel M Behar
Mar 8, 2011·The Journal of Allergy and Clinical Immunology·Mübeccel AkdisCezmi A Akdis
May 25, 2011·The Journal of Experimental Medicine·Amy E MoranKristin A Hogquist
Mar 1, 2012·PLoS Pathogens·Andrzej PawlowskiGunilla Källenius
Dec 13, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Angela M GreenJoanne L Flynn
Mar 15, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pushpa JayaramanSamuel M Behar
Jul 3, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Smita Srivastava, Joel D Ernst
Jan 7, 2014·PLoS Pathogens·Alissa C RothchildSamuel M Behar

❮ Previous
Next ❯

Citations

Feb 23, 2019·Expert Review of Vaccines·Claudio CounoupasWarwick J Britton
May 28, 2019·Immunology and Cell Biology·Claudio Counoupas, James A Triccas
May 16, 2019·Current Protocols in Immunology·Yuelin KongMaria Bettini
Apr 9, 2020·Frontiers in Immunology·Jil M JubelFrank A Schildberg
Jun 9, 2020·Frontiers in Immunology·Sajid NadeemJaved N Agrewala
Jul 8, 2020·Nature Methods·Alok V Joglekar, Guideng Li
Feb 3, 2021·The Journal of Clinical Investigation·W Henry BoomJacqueline M Achkar

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transgenic
electrophoresis
ELISA
flow cytometry
flow

Software Mentioned

COR ImageStudio
HiFi
LI
NexGen
IMANAPAM
FlowJo
HiFi Assembly

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.