Mycobacterium tuberculosis-Specific IL-21+IFN-γ+CD4+ T Cells Are Regulated by IL-12

PloS One
Li LiChangyou Wu

Abstract

In the current study of Mycobacterium tuberculosis (MTB)-specific T and B cells, we found that MTB-specific peptides from early secreted antigenic target-6 (ESAT-6) and culture filtrate protein-10 (CFP-10) induced the expression of IL-21 predominantly in CD4(+) T cells. A fraction of IL-21-expressing CD4(+) T cells simultaneously expressed Th1 cytokines but did not secrete Th2 or Th17 cytokines, suggesting that MTB-specific IL-21-expressing CD4(+) T cells were different from Th1, Th2 and Th17 subpopulations. The majority of MTB-specific IL-21-expressing CD4(+) T cells co-expressed IFN-γ and IL-21+IFN-γ(+)CD4(+) T cells exhibited obviously polyfunctionality. In addition, MTB-specific IL-21-expressing CD4(+) T cells displayed a CD45RO+CD62Ll(ow)CCR7(low)CD40L(high)ICOS(high) phenotype. Bcl-6-expression was significantly higher in IL-21-expressing CD4(+) T cells than IL-21-CD4(+) T cells. Moreover, IL-12 could up-regulate MTB-specific IL-21 expression, especially the frequency of IL-21(+)IFN-γ+CD4(+) T cells. Taken together, our results demonstrated that MTB-specific IL-21(+)IFN-γ(+)CD4(+) T cells from local sites of tuberculosis (TB) infection could be enhanced by IL-12, which have the features of both Tfh and Th1 cells and may h...Continue Reading

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Citations

Sep 1, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Satyanarayana Swamy CheekatlaRamakrishna Vankayalapati
Nov 28, 2017·Cellular & Molecular Immunology·Hongbo Shen, Zheng W Chen
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Jun 10, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tony W NgSteven A Porcelli
Jun 30, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xingyu LiYongjian Wu

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Methods Mentioned

BETA
PMA
fluorescence-activated cell sorting
FACS
biopsy

Software Mentioned

Bio
GraphPad Prism
1D
FlowJo

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