Mycobacterium tuberculosis strains disrupted in mce3 and mce4 operons are attenuated in mice

Journal of Medical Microbiology
Ryan H SenaratneL W Riley

Abstract

The Mycobacterium tuberculosis genome contains four copies of an operon called mce (mce1-4). Previously we reported that M. tuberculosis disrupted in the mce1 operon is more virulent than wild-type M. tuberculosis in mice. We generated single deletion mutants in mce3 (Deltamce3) and mce4 (Deltamce4) operons and a double deletion mutant (Deltamce3/4). Similar doubling times and growth characteristics were observed for all mutants and the wild-type (parent) M. tuberculosis H37Rv strain in culture and in macrophages. In addition, similar bacterial burdens were detected in organs from mice infected with Deltamce3 and the parent strain. However, the bacterial burdens of mice infected with Deltamce4 and Deltamce 3/4 were less than those of mice infected with the parent strain. The median survival times of mice infected with wild-type M. tuberculosis, Deltamce3, Deltamce4 and Deltamce3/4 were 40.5, 46, 58 and 62 weeks, respectively. Histopathological examination of lungs at 15 weeks post-infection showed that the extent of the lung lesions was less prominent in mice infected with Deltamce4 and Deltamce 3/4 mutants than in mice infected with the other two strains. These observations suggest that the mce3 and mce4 operons have a role di...Continue Reading

References

May 16, 1998·Trends in Microbiology·N M ParrishW R Bishai
Mar 1, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·F TekaiaS T Cole
Mar 16, 2002·Science·Christopher DyeMario C Raviglione
Oct 9, 2002·Microbiology·María P SantangeloFabiana Bigi
Sep 23, 2003·Infection and Immunity·Ashwani KumarVani Brahmachari
Dec 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nobuyuki ShimonoLee W Riley
Apr 5, 2005·Microbes and Infection·Andrea GioffréFabiana Bigi
Dec 31, 2005·Journal of Bacteriology·Nicola CasaliLee W Riley
Jul 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Swati M JoshiChristopher M Sassetti

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Citations

Jul 1, 2011·The Journal of Microbiology·Olivera MarjanovicLee W Riley
Apr 30, 2013·Tuberculosis·Nisha RathorMridula Bose
Apr 17, 2010·The Journal of Infectious Diseases·Noton K DuttaDeepak Kaushal
Nov 29, 2013·Genome Announcements·Christiane NishibeFlábio Ribeiro Araújo
Oct 31, 2012·Expert Review of Anti-infective Therapy·Rajesh JayachandranJean Pieters
Feb 23, 2012·Microbes and Infection·Laura I KleppHéctor R Morbidoni
Jul 1, 2015·Virulence·Gerald Larrouy-Maumus
Jun 13, 2014·Annals of the New York Academy of Sciences·Khisimuzi MdluliAnna Upton
Feb 25, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Wu LiJianping Xie
Mar 13, 2014·Critical Reviews in Biochemistry and Molecular Biology·Matthew F WippermanSuzanne T Thomas
Oct 19, 2012·Virulence·Marina A ForrelladFabiana Bigi
Apr 26, 2011·Vaccine·Andrés Obregón-HenaoFabiana Bigi
Oct 24, 2014·Frontiers in Immunology·Somdeb BoseDasgupta, Jean Pieters
Nov 5, 2016·International Journal of Biological Macromolecules·Shagufta KhanFaizan Ahmad
Apr 28, 2017·Environmental Microbiology·Julia García-FernándezJosé L García
Jan 19, 2018·Journal of Leukocyte Biology·Michael D StutzMarc Pellegrini
Dec 22, 2016·British Journal of Pharmacology·Areej Abuhammad
Aug 27, 2015·World Journal of Experimental Medicine·Remo GeorgeM Tino Unlap
Jul 23, 2014·Infection and Immunity·Mushtaq MirRobert N Husson
Jan 25, 2011·Molecular and Cellular Biochemistry·Fan Zhang, Jian-Ping Xie
Jan 30, 2018·Frontiers in Cellular and Infection Microbiology·Elena Cardenal-MuñozThierry Soldati
Oct 29, 2008·The Journal of Biological Chemistry·William W MohnLindsay D Eltis

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