Mycobacterium tuberculosis TetR family transcriptional regulator Rv1019 is a negative regulator of the mfd-mazG operon encoding DNA repair proteins.

FEBS Letters
Akhil Raj PushparajanRamakrishnan Ajay Kumar

Abstract

Rv1019, a member of an uncharacterized tetracycline resistance regulator family of transcriptional regulators of Mycobacterium tuberculosis H37Rv, was found to be differentially expressed during dormancy and reactivation in vitro. In this study, we show that this protein binds to its own promoter and acts as a negative regulator of its own expression. It forms dimers in vitro which is essential for the DNA binding activity. We also show that Rv1019 and downstream genes Rv1020 (mfd) and Rv1021 (mazG) are cotranscribed. Constitutive expression of Rv1019 in M. smegmatis downregulated MSMEG_5423 (mfd) and MSMEG_5422 (mazG), suggesting that Rv1019 negatively regulates these downstream genes which encode key proteins involved in DNA repair. M. smegmatis expressing Rv1019 was found to be sensitive to DNA-damaging environments, suggesting its role in regulating the DNA damage response in mycobacterium.

References

Aug 15, 2000·Angewandte Chemie·W SaengerW Hinrichs
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 10, 2003·Developmental Cell·Gustavo E SchujmanDiego de Mendoza
Apr 6, 2004·Current Opinion in Microbiology·Jeffrey Roberts, Joo-Seop Park
Feb 25, 2005·Antimicrobial Agents and Chemotherapy·Jun LinQijing Zhang
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Juan L RamosRaquel Tobes
Jul 24, 2007·Biochemical and Biophysical Research Communications·Chih-Chia SuEdward W Yu
Jul 27, 2007·Nucleic Acids Research·P RobackR Rutherford
Jul 28, 2007·BMC Infectious Diseases·Dennis J Murphy, James R Brown
Oct 30, 2007·Journal of Bacteriology·Brandan Hillerich, Janet Westpheling
Apr 25, 2009·Cellular Microbiology·Tige R RustadDavid R Sherman
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Aug 13, 2010·Nucleic Acids Research·Lars F WestbladeSeth A Darst
Jan 22, 2013·Tuberculosis·Jocelyne M LewBruno Sobral
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Leslie Cuthbertson, Justin R Nodwell
Dec 18, 2013·PLoS Pathogens·Liang-Dong LyuGuo-Ping Zhao
Sep 19, 2014·Nucleic Acids Research·Eliza J R PetersonNitin S Baliga
May 31, 2015·Molecular & Cellular Proteomics : MCP·Vipin GopinathRamakrishnan Ajay Kumar
Jul 28, 2016·Tuberculosis·Shaaretha PellyGyanu Lamichhane
Feb 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Xiao-Yong FanLiang-Dong Lyu
Nov 20, 2018·Molecular Cell·Mark N RaghebHoura Merrikh

❮ Previous
Next ❯

Citations

Feb 16, 2021·Microbiological Research·Shuang HanJianping Xie

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.