Mycobiome changes in the vitreous of post fever retinitis patients

PloS One
Kotakonda ArunasriSisinthy Shivaji

Abstract

Fungi have been associated with various diseases of the eye like keratitis, uveitis and endophthalmitis. Despite this fact, fungal microbiome (mycobiome) studies compared to the bacterial microbiome studies have remained neglected. In the present study, using metagenomic sequencing, the mycobiomes of the vitreous of healthy control individuals (VC, n = 15) and individuals with post fever retinitis + non-PFR uveitis (PFR+, n = 9) were analysed and compared. The results indicated that Ascomycota was the most predominant phylum in both VC and PFR+ groups. Further, at the genera level it was observed that the abundance of 17 fungal genera were significantly different in post fever retinitis (PFR, n = 6) group compared to control group. Of these 17 genera, it was observed that 14 genera were relatively more abundant in PFR group and the remaining 3 genera in the VC group. Genus Saccharomyces, a commensal of the gut and skin, was predominantly present in the vitreous of both the cohorts, however it was significantly less abundant in PFR group. Further, significant increase in the genera that have a pathogenic interaction with the host were observed in PFR group. On the whole the mycobiome in both the groups differed significantly and...Continue Reading

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Citations

Feb 8, 2021·Experimental Eye Research·Konduri RanjithSisinthy Shivaji

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Datasets Mentioned

BETA
PRJNA646315

Methods Mentioned

BETA
scrapings
biopsy
PCR

Software Mentioned

R
FastQC
Bowtie 2
GraphPad Prism
Cutadapt
trim
ade4 package
CoNet
Cytoscape
vegan

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