Mycofabricated biosilver nanoparticles interrupt Pseudomonas aeruginosa quorum sensing systems

Scientific Reports
Braj R SinghHarikesh B Singh

Abstract

Quorum sensing (QS) is a chemical communication process that Pseudomonas aeruginosa uses to regulate virulence and biofilm formation. Disabling of QS is an emerging approach for combating its pathogenicity. Silver nanoparticles (AgNPs) have been widely applied as antimicrobial agents against human pathogenic bacteria and fungi, but not for the attenuation of bacterial QS. Here we mycofabricated AgNPs (mfAgNPs) using metabolites of soil fungus Rhizopus arrhizus BRS-07 and tested their effect on QS-regulated virulence and biofilm formation of P. aeruginosa. Transcriptional studies demonstrated that mfAgNPs reduced the levels of LasIR-RhlIR. Treatment of mfAgNPs inhibited biofilm formation, production of several virulence factors (e.g. LasA protease, LasB elastrase, pyocyanin, pyoverdin, pyochelin, rhamnolipid, and alginate) and reduced AHLs production. Further genes quantification analyses revealed that mfAgNPs significantly down-regulated QS-regulated genes, specifically those encoded to the secretion of virulence factors. The results clearly indicated the anti-virulence property of mfAgNPs by inhibiting P. aeruginosa QS signaling.

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Citations

Oct 26, 2016·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Cristina Prat, Alicia Lacoma
Jun 27, 2017·Journal of Applied Microbiology·K Naik, M Kowshik
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Methods Mentioned

BETA
surface plasmon resonance
X-ray
scanning electron microscopy
transmission electron microscopy
infrared spectroscopy

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