PMID: 8962147Dec 10, 1996Paper

Mycoparasitic interaction relieves binding of the Cre1 carbon catabolite repressor protein to promoter sequences of the ech42 (endochitinase-encoding) gene in Trichoderma harzianum

Proceedings of the National Academy of Sciences of the United States of America
Matteo LoritoChristian P Kubicek

Abstract

The fungus Trichoderma harzianum is a potent mycoparasite of various plant pathogenic fungi. We have studied the molecular regulation of mycoparasitism in the host/mycoparasite system Botrytis cinerea/T. harzianum. Protein extracts, prepared from various stages of mycoparasitism, were used in electrophoretic mobility-shift assays (EM-SAs) with two promoter fragments of the ech-42 (42-kDa endochitinase-encoding) gene of T. harzianum. This gene was chosen as a model because its expression is triggered during mycoparasitic interaction [Carsolio, C., Gutierrez, A., Jimenez, B., van Montagu, M. & Herrera-Estrella, A. (1994) Proc. Natl. Acad. Sci. USA 91, 10903-10907]. All cell-free extracts formed high-molecular weight protein-DNA complexes, but those obtained from mycelia activated for mycoparasitic attack formed a complex with greater mobility. Competition experiments, using oligonucleotides containing functional and nonfunctional consensus sites for binding of the carbon catabolite repressor Cre1, provided evidence that the complex from nonmycoparasitic mycelia involves the binding of Cre1 to both fragments of the ech-42 promoter. The presence of two and three consensus sites for binding of Cre1 in the two ech-42 promoter fragmen...Continue Reading

References

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Citations

Feb 1, 1993·Enzyme and Microbial Technology·C P KubicekE M Kubicek-Pranz
Jul 3, 1998·Current Opinion in Biotechnology·R Cohen-Kupiec, I Chet
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Karl-Heinz KogelUwe Sonnewald
May 3, 2011·Applied and Environmental Microbiology·Barbara ReithnerAlfredo Herrera-Estrella
Jan 12, 2007·Canadian Journal of Microbiology·D C MorissetteS H Jabaji-Hare
Oct 8, 2011·PloS One·Jianping Sun, N Louise Glass
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·M LoritoI G Fernandez
Jul 14, 2009·Applied Biochemistry and Biotechnology·Marcelo Fernando KernGiancarlo Pasquali
Nov 7, 2006·Journal of Microbiological Methods·Sébastien Massart, Haissam M Jijakli
Jun 30, 2012·Proteomics·Luis H F Do ValeMarcelo V Sousa
Mar 20, 2010·Environmental Microbiology·Cheng-Gang ZouKe-Qin Zhang
Nov 17, 2009·Molecular Microbiology·Wilfried Jonkers, Martijn Rep
Feb 23, 2007·FEMS Microbiology Reviews·Kirstee MartinRobert J Seviour
Sep 21, 2007·Fungal Genetics and Biology : FG & B·Miguel A Moreno-MateosTahía Benítez
Jan 4, 2018·International Journal of Molecular Sciences·Muhammad AdnanGuodong Lu
Feb 13, 2016·Microbiology and Molecular Biology Reviews : MMBR·Monika SchmollAlfredo Herrera-Estrella
Feb 28, 2007·Medical Mycology·Michael R McGinnis
Jun 16, 2017·Biotechnology for Biofuels·Tiziano BenocciRonald P de Vries
Mar 5, 2005·Applied and Environmental Microbiology·Susanne ZeilingerRobert L Mach
Mar 5, 2005·Applied and Environmental Microbiology·Brian BoyleArmand Séguin
Dec 20, 2017·Molecular Plant-microbe Interactions : MPMI·Claudia A Ramírez-ValdespinoVianey Olmedo-Monfil
Jun 9, 2006·Mycopathologia·Li Duo-Chuan

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